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Orienting DNA Helices on Gold Using Applied Electric Fields

Kelley, Shana O. and Barton, Jacqueline K. and Jackson, Nicole M. and McPherson, Lee D. and Potter, Aaron B. and Spain, Eileen M. and Allen, Michael J. and Hill, Michael G. (1998) Orienting DNA Helices on Gold Using Applied Electric Fields. Langmuir, 14 (24). pp. 6781-6784. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20180719-155943234

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Abstract

Gold surfaces modified with thiol-derivatized DNA duplexes have been investigated as a function of applied electrochemical potential via atomic force microscopy (EC-AFM). At open circuit, monolayers of well-packed DNA helices form with a film depth of 45(3) Å. On the basis of the anisotropic dimensions of these 15 base pair duplexes (20 Å in diameter versus 50 Å in length), this corresponds to an average ∼45° orientation of the helical axis with respect to the gold surface. Under potential control, the monolayer thickness (and therefore the orientation of the helices) changes dramatically with applied potential. At potentials negative of ∼0.45 V (versus a Ag wire quasi-reference electrode) film thicknesses of ∼55 Å are observed, whereas at more positive potentials the monolayer thickness drops to a limiting value of ∼20 Å. These results are consistent with a morphology change in which the helices either stand straight up or lie flat down on the metal surface, depending on the electrode potential relative to the potential of zero charge (pzc). This voltage-induced morphology change is reversible and effectively constitutes a nanoscale mechanical “switch”.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dx.doi.org/10.1021/la980874nDOIArticle
ORCID:
AuthorORCID
Kelley, Shana O.0000-0003-3360-5359
Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 1998 American Chemical Society. Received July 14, 1998. In Final Form: September 24, 1998. This work was supported by the NSF (M.G.H. and E.M.S.), the Research Corporation (M.G.H.), and the NIH (Grant GM49216 to J.K.B., predoctoral traineeship to S.O.K.).
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Research CorporationUNSPECIFIED
NIHGM49216
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:24
Record Number:CaltechAUTHORS:20180719-155943234
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180719-155943234
Official Citation:Orienting DNA Helices on Gold Using Applied Electric Fields Shana O. Kelley, Jacqueline K. Barton, Nicole M. Jackson, Lee D. McPherson, Aaron B. Potter, Eileen M. Spain, Michael J. Allen, and Michael G. Hill Langmuir 1998 14 (24), 6781-6784 DOI: 10.1021/la980874n
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88036
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jul 2018 23:09
Last Modified:03 Oct 2019 20:02

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