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Diacetylene Chelator Lipids as Support for Immobilization and Imaging of Proteins by Atomic Force Microscopy

Dorn, Ingmar T. and Hofmann, Ulrich G. and Peltonen, Jouko and Tampé, Robert (1998) Diacetylene Chelator Lipids as Support for Immobilization and Imaging of Proteins by Atomic Force Microscopy. Langmuir, 14 (17). pp. 4836-4842. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20180719-160429047

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Abstract

Chelator lipids represent a powerful and flexible tool to immobilize, orient, and crystallize histidine-tagged proteins at interfaces. To produce stable two-dimensional polymers that are biofunctional, we synthesized diacetylene lipids carrying a metal-chelating headgroup. These lipids were characterized at the air−water interface with respect to their thermodynamic properties, complex formation, and photopolymerization using film balance techniques combined with epi-fluorescence microscopy. Polymerized monolayers were transferred onto solid supports and reversible binding of histidine-tagged protein/DNA complexes was followed by atomic force microscopy. The versatility of the chelator lipid concept may open the possibility to examine structure and function of proteins or multiprotein assemblies under native conditions and in real time by scanning probe microscopy.


Item Type:Article
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https://dx.doi.org/10.1021/la980284pDOIArticle
Additional Information:© 1998 American Chemical Society. Received March 10, 1998. In Final Form: May 19, 1998. We thank Hermann Gaub, Walter Häckl, Hans Ribi, Jarl Rosenholm, Erich Sackmann, Lutz Schmitt, and Rudolf Simson for helpful discussions, as well as Wolfram Schäfer and Isolde Sonnenbichler for providing the MS and NMR spectra. We grateful acknowledge Oliver Boscheinen and Klaus-Dieter Scharf for supplying the HSF/DNA complex. This work was supported by the Deutsche Forschungsgemeinschaft and the European Commission (BIOTECH program).
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Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
European CommissionUNSPECIFIED
Issue or Number:17
Record Number:CaltechAUTHORS:20180719-160429047
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180719-160429047
Official Citation:Diacetylene Chelator Lipids as Support for Immobilization and Imaging of Proteins by Atomic Force Microscopy Ingmar T. Dorn, Ulrich G. Hofmann, Jouko Peltonen, and Robert Tampé Langmuir 1998 14 (17), 4836-4842 DOI: 10.1021/la980284p
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88037
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jul 2018 23:11
Last Modified:03 Oct 2019 20:02

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