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Surface Site Heterogeneity and Lateral Interactions in Multipoint Protein Adsorption

Johnson, R. D. and Wang, Z.-G. and Arnold, F. H. (1996) Surface Site Heterogeneity and Lateral Interactions in Multipoint Protein Adsorption. Journal of Physical Chemistry, 100 (12). pp. 5134-5139. ISSN 0022-3654 . https://resolver.caltech.edu/CaltechAUTHORS:20180720-141711681

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Abstract

Studies carried out using engineered proteins have demonstrated that protein adsorption to functional surfaces involves multiple interactions between specific groups on the protein and complementary binding sites distributed on the surface. A consequence of multipoint interactions is that protein binding affinity should depend strongly on the distribution of surface binding sites. In this investigation we present a thermodynamic framework for multipoint protein binding to a random arrangement of surface binding sites that also includes lateral interactions among adsorbed protein molecules. This framework results in reversible adsorption behavior analogous to that predicted by the Temkin model and chromatographic behavior analogous to that predicted by the “stoichiometric displacement” model (SDM). Using this framework we can now interpret the semiempirical parameters obtained using these models for protein binding in chromatographic systems in terms of thermodynamic parameters for protein−surface interactions. We show a correlation between Temkin model parameters for a series of cytochrome c variants in immobilized metal affinity chromatography (IMAC) that is consistent with protein adsorption to a nonuniform arrangement of surface binding sites. Lateral interactions among adsorbed protein molecules are shown to be insignificant for this system.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://dx.doi.org/10.1021/jp9523682DOIArticle
ORCID:
AuthorORCID
Wang, Z.-G.0000-0002-3361-6114
Arnold, F. H.0000-0002-4027-364X
Additional Information:© 1996 American Chemical Society. Received: August 15, 1995; In Final Form: NoVember 1, 1995. This research is supported by the National Science Foundation. F.H.A. acknowledges an NSF PYI award and a fellowship from the David and Lucile Packard Foundation. R.D.J. acknowledges a predoctoral training fellowship from the U. S. National Institute of General Medical Sciences, Pharmacology Sciences Program.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:12
Record Number:CaltechAUTHORS:20180720-141711681
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180720-141711681
Official Citation:Surface Site Heterogeneity and Lateral Interactions in Multipoint Protein Adsorption R. D. Johnson, Z.-G. Wang, and F. H. Arnold The Journal of Physical Chemistry 1996 100 (12), 5134-5139 DOI: 10.1021/jp9523682
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88080
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Jul 2018 20:24
Last Modified:03 Oct 2019 20:02

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