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The Reactions of Antiserum Homologous to the p-Azosuccinanilate Ion Group

Pressman, David and Bryden, John H. and Pauling, Linus (1948) The Reactions of Antiserum Homologous to the p-Azosuccinanilate Ion Group. Journal of the American Chemical Society, 70 (4). pp. 1352-1358. ISSN 0002-7863. doi:10.1021/ja01184a021.

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It was discovered by Landsteiner and van der Scheer that the precipitation of azoprotein containing the p-azosuccinanilate ion haptenic group by hapten-homologous antiserum (anti-S_p serum) is inhibited just as well by maleate ion as by succinate ion, whereas fumarate ion is practically ineffective, and from this observation the cautious conclusion was drawn that “Accordingly, one could suppose that the succinic acid molecule can exist in a form corresponding to the cis configuration, or that the antibodies adjust themselves to this.” Because of our interest in the use of immunochemical techniques for the determination of the configuration of molecules and haptenic groups, we have extended our quantitative studies of hapten inhibition of serological precipitation to include the S_p system, and have investigated the effect of over fifty haptens on the precipitation of S_p-ovalbumin and anti-S_p serum. The analysis of the data has shown that the normal configuration of the p-azosuccinanilate ion group in aqueous solution is a cis configuration, presumably stabilized by a hydrogen bond, and has provided information about the configuration of other ions.

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Additional Information:© 1948 American Chemical Society. Received October 14, 1947. This investigation was carried out with the aid of a grant from The Rockefeller Foundation. We wish to thank Mr. Dan Rice for assisting in the analytical work.
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Rockefeller FoundationUNSPECIFIED
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Gates and Crellin Laboratories of Chemistry1154
Issue or Number:4
Record Number:CaltechAUTHORS:20180724-121639840
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Official Citation:The Reactions of Antiserum Homologous to the p-Azosuccinanilate Ion Group David Pressman, John H. Bryden, and Linus Pauling Journal of the American Chemical Society 1948 70 (4), 1352-1358 DOI: 10.1021/ja01184a021
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88186
Deposited By: George Porter
Deposited On:24 Jul 2018 21:39
Last Modified:16 Nov 2021 00:24

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