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The use of isotopic carbon in a study of the metabolism of anthanilic acid in Neurospora

Nyc, Joseph F. and Mitchell, Herschel K. and Leifer, Edgar and Langham, Wright H. (1949) The use of isotopic carbon in a study of the metabolism of anthanilic acid in Neurospora. Journal of Biological Chemistry, 179 (2). pp. 783-787. ISSN 0021-9258.

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The finding by Tatum, Banner, and Beadle (l), that the tryptophanless Neurospora mutant strain 10575 accumulates anthranilic acid, which in turn can be utilized for growth of strain 40008, has provided evidence that anthranilic acid is a biochemical precursor of tryptophan in this organism. It has been further established that indole is an intermediate in this conversion (2-5). More recent work with a number of mutants of Neurospora (6-8) has established that tryptophan is a biochemical precursor to niacin with kynurenine and hydroxyanthranilic acid as intermediates. The accumulated evidence has indicated the existence in the mold of the following series of reactions: → Anthranilic acid → indole → tryptophan → kynurenine → 3-hydroxyanthranilic acid → nicotinic acid In the light of this evidence the present work was undertaken to trace the carbon in the carboxyl group of anthranilic acid in order to estimate its contribution as a structural unit in the formation of niacin and tryptophan. The organism chosen for this investigation was a biochemical mutant strain of Neurospora designated as strain 40008. This mutant utilizes anthranilic acid, indole, or tryptophan for growth. The mutant was grown in the presence of anthranilic acid containing Cl4 in the carboxyl group. Niacin and tryptophan were isolated from the mold mycelium and tested for radioactivity.

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Additional Information:Copyright © 1949 by the American Society for Biochemistry. (Received for publication, January 31, 1949) These investigations were supported in part by the Williams-Waterman Fund for the Combat of Dietary Diseases and the Rockefeller Foundation. They were also carried out as a part of the work done under contract No. N-6onr-944, Task Order 5, United States Navy Department, and contract No. W-7495-eng-36 for the Atomic Energy Commission.
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8822
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Deposited On:19 Sep 2007
Last Modified:02 Oct 2019 23:54

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