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A Quantitative Study of the Hydrolysis of Human Dinitrophenyl(DNP)globin: The Number and Kind of Polypeptide Chains in Normal Adult Human Hemoglobin

Rhinesmith, Herbert S. and Schroeder, W. A. and Pauling, Linus (1957) A Quantitative Study of the Hydrolysis of Human Dinitrophenyl(DNP)globin: The Number and Kind of Polypeptide Chains in Normal Adult Human Hemoglobin. Journal of the American Chemical Society, 79 (17). pp. 4682-4686. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20180725-111211357

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Abstract

A quantitative investigation of the partial hydrolysis of DNP-globin in refluxing 6 N hydrochloric acid has led to an explanation of our earlier conclusion that normal adult human hemoglobin contains a non-integral number, 3.6, of N-terminal valyl residues per molecule. It is now concluded that 4 E-terminal residues are present. Moreover, it has been found that the molecule contains two kinds of polypeptide chains, with respect to the K-termini. Under the above hydrolytic conditions the N-terminal valyl residues are released as DNP-Val-leu almost quantitatively from two chains (A chains) within 15 min. On continued hydrolysis the other two chains (B chains) release DNP-valine. No N-terminal peptides originating from the B chains have been definitely identified.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/ja01574a028DOIArticle
Additional Information:© 1957 American Chemical Society. Received April 11, 1957. This investigation was supported in part by a grant from the Ford Foundation and in part by a research grant (RG-4276(C)) from the National Institutes of Health, Public Health Service.
Funders:
Funding AgencyGrant Number
Ford FoundationUNSPECIFIED
NIHRG-4276(C)
Other Numbering System:
Other Numbering System NameOther Numbering System ID
Gates and Crellin Laboratories of Chemistry2199
Record Number:CaltechAUTHORS:20180725-111211357
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180725-111211357
Official Citation:A Quantitative Study of the Hydrolysis of Human Dinitrophenyl(DNP)globin: The Number and Kind of Polypeptide Chains in Normal Adult Human Hemoglobin Herbert S. Rhinesmith, W. A. Schroeder, and Linus Pauling Journal of the American Chemical Society 1957 79 (17), 4682-4686 DOI: 10.1021/ja01574a028
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88266
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:25 Jul 2018 20:08
Last Modified:25 Jul 2018 20:08

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