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Aberrant splicing of proteolipid protein mRNA in the dysmyelinating jimpy mutant mouse

Hudson, Lynn D. and Berndt, Jo Ann and Puckett, Carmie and Kozak, Christine A. and Lazzarini, Robert A. (1987) Aberrant splicing of proteolipid protein mRNA in the dysmyelinating jimpy mutant mouse. Proceedings of the National Academy of Sciences of the United States of America, 84 (5). pp. 1454-1458. ISSN 0027-8424. PMCID PMC304449. doi:10.1073/pnas.84.5.1454.

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cDNA clones encoding proteolipid protein (PLP) were isolated from a mouse brain library and sequenced. We describe two transcripts arising from the PLP locus by alternative splicing: the major one encodes the 277-amino acid PLP protein and the minor one corresponds to the DM-20 protein, a PLP-like protein of 20,000 Mr that shares both amino and carboxyl regions with PLP. These two transcripts lack ≈70 bases in PLP mRNA from the dysmyelinating jimpy mutant. The deletion spans amino acids 208-232; however, this region is present in the jimpy PLP-encoding gene. We propose that the jimpy mutant suffers a point mutation or the deletion of a few bases in the PLP gene that alters the normal splicing pattern and generates partially deleted PLP transcripts.

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Additional Information:© 1987 by the National Academy of Sciences. Communicated by DeWitt Stetten, Jr., November 10, 1986. We thank Drs. S. Billings-Gagliardi and M.K. Wolf for the gift of mutant mice, Dr. M. Brownstein for supplying synthetic oligonucleotides, Dr. N. Zeller for helpful discussions, Blanche Lewis for technical assistance, Don Johnson for computer expertise, and Charlene French for expert manuscript preparation. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:oligodendrocyte maturation; myelin; DM-20
Issue or Number:5
PubMed Central ID:PMC304449
Record Number:CaltechAUTHORS:HUDpnas87
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8838
Deposited By: Tony Diaz
Deposited On:20 Sep 2007
Last Modified:08 Nov 2021 20:53

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