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HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers

Zhou, Yun and Yan, An and Han, Han and Li, Ting and Geng, Yuan and Liu, Xing and Meyerowitz, Elliot M. (2018) HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers. Science, 361 (6401). pp. 502-506. ISSN 0036-8075. PMCID PMC6095697. https://resolver.caltech.edu/CaltechAUTHORS:20180802-135135697

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Abstract

The control of the location and activity of stem cells depends on spatial regulation of gene activities in the stem cell niche. Using computational and experimental approaches, we have tested and found support for a hypothesis for gene interactions that specify the Arabidopsis apical stem cell population. The hypothesis explains how the WUSCHEL gene product, synthesized basally in the meristem, induces CLAVATA3-expressing stem cells in the meristem apex but, paradoxically, not in the basal domain where WUSCHEL itself is expressed. The answer involves the activity of the small family of HAIRY MERISTEM genes, which prevent the activation of CLAVATA3 and which are expressed basally in the shoot meristem.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.aar8638DOIArticle
https://www.sciencemag.org/content/361/6401/502/suppl/DC1PublisherSupplementary Materials
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6095697PubMed CentralArticle
ORCID:
AuthorORCID
Yan, An0000-0002-4676-5442
Li, Ting0000-0001-8273-5593
Liu, Xing0000-0002-0506-6821
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2018 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 26 December 2017; accepted 19 June 2018. We are grateful to H. Yang for sharing the HAM1m DNA construct and to A. Garda from Caltech and C. Layug from Purdue for technical support. The work at Caltech was funded by NIH grant R01 GM104244 and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through grant GBMF3406 to E.M.M.). The work at Purdue was funded by a start-up package and support from the Purdue Center for Plant Biology to Y.Z. Author contributions: Y.Z., A.Y., and E.M.M. conceived of the research direction; Y.Z., H.H., T.L., and Y.G. performed experiments; A.Y. performed modeling and simulation; Y.Z., X.L., and E.M.M. commented on the model; X.L. contributed reagents; Y.Z., A.Y., and X.L. analyzed and interpreted data; Y.Z. and E.M.M. supervised the project; Y.Z., A.Y., X.L., and E.M.M. wrote the manuscript; and H.H., T.L., and Y.G. commented on the manuscript. The authors declare that they have no competing interests. Data and materials availability: All data are available in the main text or the supplementary materials.
Funders:
Funding AgencyGrant Number
NIHR01 GM104244
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationGBMF3406
Purdue UniversityUNSPECIFIED
Issue or Number:6401
PubMed Central ID:PMC6095697
Record Number:CaltechAUTHORS:20180802-135135697
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180802-135135697
Official Citation:HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers. BY YUN ZHOU, AN YAN, HAN HAN, TING LI, YUAN GENG, XING LIU, ELLIOT M. MEYEROWITZ. SCIENCE 03 AUG 2018: 502-506
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88506
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:02 Aug 2018 21:06
Last Modified:10 Apr 2020 19:53

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