Zhou, Yun and Yan, An and Han, Han and Li, Ting and Geng, Yuan and Liu, Xing and Meyerowitz, Elliot M. (2018) HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers. Science, 361 (6401). pp. 502-506. ISSN 0036-8075. PMCID PMC6095697. doi:10.1126/science.aar8638. https://resolver.caltech.edu/CaltechAUTHORS:20180802-135135697
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Abstract
The control of the location and activity of stem cells depends on spatial regulation of gene activities in the stem cell niche. Using computational and experimental approaches, we have tested and found support for a hypothesis for gene interactions that specify the Arabidopsis apical stem cell population. The hypothesis explains how the WUSCHEL gene product, synthesized basally in the meristem, induces CLAVATA3-expressing stem cells in the meristem apex but, paradoxically, not in the basal domain where WUSCHEL itself is expressed. The answer involves the activity of the small family of HAIRY MERISTEM genes, which prevent the activation of CLAVATA3 and which are expressed basally in the shoot meristem.
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| Additional Information: | © 2018 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 26 December 2017; accepted 19 June 2018. We are grateful to H. Yang for sharing the HAM1m DNA construct and to A. Garda from Caltech and C. Layug from Purdue for technical support. The work at Caltech was funded by NIH grant R01 GM104244 and by the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation (through grant GBMF3406 to E.M.M.). The work at Purdue was funded by a start-up package and support from the Purdue Center for Plant Biology to Y.Z. Author contributions: Y.Z., A.Y., and E.M.M. conceived of the research direction; Y.Z., H.H., T.L., and Y.G. performed experiments; A.Y. performed modeling and simulation; Y.Z., X.L., and E.M.M. commented on the model; X.L. contributed reagents; Y.Z., A.Y., and X.L. analyzed and interpreted data; Y.Z. and E.M.M. supervised the project; Y.Z., A.Y., X.L., and E.M.M. wrote the manuscript; and H.H., T.L., and Y.G. commented on the manuscript. The authors declare that they have no competing interests. Data and materials availability: All data are available in the main text or the supplementary materials. | ||||||||||||
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| Issue or Number: | 6401 | ||||||||||||
| PubMed Central ID: | PMC6095697 | ||||||||||||
| DOI: | 10.1126/science.aar8638 | ||||||||||||
| Record Number: | CaltechAUTHORS:20180802-135135697 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180802-135135697 | ||||||||||||
| Official Citation: | HAIRY MERISTEM with WUSCHEL confines CLAVATA3 expression to the outer apical meristem layers. BY YUN ZHOU, AN YAN, HAN HAN, TING LI, YUAN GENG, XING LIU, ELLIOT M. MEYEROWITZ. SCIENCE 03 AUG 2018: 502-506 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 88506 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||
| Deposited On: | 02 Aug 2018 21:06 | ||||||||||||
| Last Modified: | 09 Mar 2022 18:30 |
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