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Biochemical assays used for in vitro and in vivo gene expression

Ahmed, M. and Narain, R. (2016) Biochemical assays used for in vitro and in vivo gene expression. In: Polymers and Nanomaterials for Gene Therapy. Woodhead Publishing , Oxford, pp. 257-270. ISBN 978-0-08-100520-0. http://resolver.caltech.edu/CaltechAUTHORS:20180806-073905160

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Abstract

Development of sensitive biochemical assays is important for the detection of diseased tissue, single nucleotide polymorphism, and gene expression analysis. Biochemical assays are mainly based on a combination of electrophoretic and hybridization techniques. Hybridization techniques are well-characterized, sensitive, and quantitative. Polymerase chain reaction, fluorescence in situ hybridization, and micro-arrays are examples of highly sensitive hybridization approaches, used in laboratories and clinics, for the detection of exogenous or diseased deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The combination of hybridization approaches with highly sensitive instruments such as mass adsorption laser desorption ionization time of flight mass spectrophotometer (MALDI-TOF MS) allows accurate detection of DNA and RNA molecules in vitro. Reporter gene systems are also frequently used in research for the indication of successful delivery of exogenous DNA. In vivo detection of DNA/RNA is cumbersome and is achieved by optical imaging, magnetic resonance imaging, or immunohistochemistry.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/B978-0-08-100520-0.00011-4DOIArticle
Additional Information:© 2016 Elsevier Ltd. Available online 19 February 2016.
Subject Keywords:Gene reporter system; polymerase chain reaction; fluorescence in situ hybridization; Micro-array; immunohistochemistry; magnetic resonance imaging
Record Number:CaltechAUTHORS:20180806-073905160
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180806-073905160
Official Citation:M. Ahmed, R. Narain, 11 - Biochemical assays used for in vitro and in vivo gene expression, Editor(s): Ravin Narain, Polymers and Nanomaterials for Gene Therapy, Woodhead Publishing, 2016, Pages 257-270, ISBN 9780081005200, https://doi.org/10.1016/B978-0-08-100520-0.00011-4. (http://www.sciencedirect.com/science/article/pii/B9780081005200000114)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88580
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Aug 2018 16:27
Last Modified:06 Aug 2018 16:27

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