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Ultraparamagnetic cells formed through intracellular oxidation and chelation of paramagnetic iron

Ramesh, Pradeep and Hwang, Son-Jong and Davis, Hunter C. and Lee-Gosselin, Audrey and Bharadwaj, Vivek and English, Max A. and Sheng, Jenny and Iyer, Vasant and Shapiro, Mikhail G. (2018) Ultraparamagnetic cells formed through intracellular oxidation and chelation of paramagnetic iron. Angewandte Chemie International Edition, 57 (38). pp. 12385-12389. ISSN 1433-7851. https://resolver.caltech.edu/CaltechAUTHORS:20180813-080049505

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Abstract

Making cells magnetic is a long‐standing goal of chemical biology, aiming to enable the separation of cells from complex biological samples and their visualization in vivo using magnetic resonance imaging (MRI). Previous efforts towards this goal, focused on engineering cells to biomineralize superparamagnetic or ferromagnetic iron oxides, have been largely unsuccessful due to the stringent required chemical conditions. Here, we introduce an alternative approach to making cells magnetic, focused on biochemically maximizing cellular paramagnetism. We show that a novel genetic construct combining the functions of ferroxidation and iron chelation enables engineered bacterial cells to accumulate iron in “ultraparamagnetic” macromolecular complexes, allowing these cells to be trapped with magnetic fields and imaged with MRI in vitro and in vivo. We characterize the properties of these cells and complexes using magnetometry, nuclear magnetic resonance, biochemical assays, and computational modeling to elucidate the unique mechanisms and capabilities of this paramagnetic concept.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/anie.201805042DOIArticle
ORCID:
AuthorORCID
Hwang, Son-Jong0000-0002-3210-466X
Davis, Hunter C.0000-0003-1655-692X
Lee-Gosselin, Audrey0000-0002-2431-2741
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Accepted manuscript online: 08 August 2018; Manuscript accepted: 07 August 2018; Manuscript revised: 06 August 2018; Manuscript received: 30 April 2018. We thank Matthew Chalkley for assistance with Mossbauer spectroscopy, Dr. Paul Oyala for assistance with magnetic resonance and Dr. Michael Tyszka for helpful discussions. This research was supported by the W.M. Keck Foundation, the Dana Foundation and the Burroughs Wellcome Fund. P.R. was supported by a National Science Foundation Graduate Research Fellowship and a National Institutes of Health training grant (5T32GM112592). Related research in the Shapiro laboratory is also supported by the Heritage Medical Research Institute, the David and Lucille Packard Foundation and the Pew Charitable Trusts. The authors declare no conflict of interest.
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
W. M. Keck FoundationUNSPECIFIED
Dana FoundationUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
NIH Predoctoral Fellowship5T32GM112592
Heritage Medical Research InstituteUNSPECIFIED
David and Lucille Packard FoundationUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
Subject Keywords:ultraparamagnetism; magnetic resonance imaging; magnetic capture; ferrogel
Issue or Number:38
Record Number:CaltechAUTHORS:20180813-080049505
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180813-080049505
Official Citation:P. Ramesh, S.-J. Hwang, H. C. Davis, A. Lee-Gosselin, V. Bharadwaj, M. A. English, J. Sheng, V. Iyer, M. G. Shapiro, Angew. Chem. Int. Ed. 2018, 57, 12385
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:88775
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Aug 2018 15:20
Last Modified:22 Nov 2019 22:45

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