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SCHEMA-Guided Protein Recombination

Silberg, Jonathan J. and Endelman, Jeffrey B. and Arnold, Frances H. (2004) SCHEMA-Guided Protein Recombination. In: Protein Engineering. Methods in Enzymology. No.388. Academic Press , San Diego, CA, pp. 35-42. ISBN 9780121827939. https://resolver.caltech.edu/CaltechAUTHORS:20180821-161122360

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Abstract

This chapter examines the different aspects of SCHEMA-guided protein recombination. SCHEMA is a scoring function that predicts which elements in homologous proteins can be swapped without disturbing the integrity of the structure. Using the structural coordinates of the parent proteins, SCHEMA identifies pairs of residues that are interacting, and determines the number of interactions that are broken when a chimeric protein inherits portions of its sequence from different parents. The SCHEMA disruption of a chimeric sequence made by recombining sequence elements from homologous proteins is presented. The best methods available for creating the libraries identified by SCHEMA as enriched in folded chimeras are sequence-independent site directed chimeragenesis and chemical synthesis. These techniques can recombine parents with any level of sequence identity at multiple sites and easily create libraries encoding thousands of chimeras. It is observed that when the parents do not exhibit sufficient identity at the desired crossover locations, synonymous mutations can often be introduced to allow recombination at that site. It is found that SCHEMA only calculates the disruption arising from recombination, not from mutation.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/S0076-6879(04)88004-2DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2004 Elsevier Inc. Available online 6 August 2004.
Series Name:Methods in Enzymology
Issue or Number:388
DOI:10.1016/S0076-6879(04)88004-2
Record Number:CaltechAUTHORS:20180821-161122360
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180821-161122360
Official Citation:Jonathan J Silberg, Jeffrey B Endelman, Frances H Arnold, SCHEMA-Guided Protein Recombination, Methods in Enzymology, Academic Press, Volume 388, 2004, Pages 35-42, ISSN 0076-6879, ISBN 9780121827939, https://doi.org/10.1016/S0076-6879(04)88004-2. (http://www.sciencedirect.com/science/article/pii/S0076687904880042)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89000
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Aug 2018 23:18
Last Modified:16 Nov 2021 00:31

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