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Molecularly imprinted polymers on silica: selective supports for high-performance ligand-exchange chromatography

Plunkett, Sean D. and Arnold, Frances H. (1995) Molecularly imprinted polymers on silica: selective supports for high-performance ligand-exchange chromatography. Journal of Chromatography A, 708 (1). pp. 19-29. ISSN 0021-9673. doi:10.1016/0021-9673(95)00378-Z.

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Thin coatings of molecularly imprinted, metal-complexing polymers have been grafted to activated silica beads suitable for high-performance liquid chromatography (HPLC). Propylmethacrylate-activated silica particles were coated by copolymerization with a metal-chelating monomer, Cu^(2+)-[N-(4-vinylbenzyl)-imino]diabetic acid, a metal-coordinating (imidazole) template, and ethylene glycol dimethacrylate. After extraction to remove the template and re-loading with metal, the composite materials re-bind the templates with which they were prepared and exhibit selectivities comparable to bulk-polymerized imprinted materials. The strong Cu^(2+)-imidazole interaction, desirable for creating a high-fidelity imprint, leads to excessive retention in elution chromatography. By replacing the copper in the imprinted metal-complexing polymers with weaker-binding Zn^(2+), these novel ligand-exchange supports can effect partial to complete chromatographic separation of their bis-imidazole templates from other, highly similar imidazole-containing substrates. This “bait-and-switch” approach can significantly enhance the performance of molecularly imprinted materials. Scatchard plots of equilibrium binding data show a significant degree of heterogeneity in the imprinted binding sites of material prepared with a bis-imidazole template, but not with a mono-imidazole template. The best chromatographic separations are observed with small sample sizes, where the substrates occupy the strongest (highest-fidelity) sites.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1995 Published by Elsevier B.V. First received 23 December 1994; revised manuscript received 17 March 1995; accepted 17 March 1995. This research is supported by the National Science Foundation (BCS-9108502) and the Office of Naval Research (N00014-92-J-1178). F.H.A. acknowledges an NSF Presidential Young Investigator Award and a fellowship from the David and Lucile Packard Foundation. S.P. acknowledges a predoctoral training fellowship in biotechnology from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01.
Funding AgencyGrant Number
Office of Naval Research (ONR)N00014-92-J-1178
David and Lucile Packard FoundationUNSPECIFIED
NIH Predoctoral Fellowship1 T32 GM 08346-01
Issue or Number:1
Record Number:CaltechAUTHORS:20180822-075236331
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Official Citation:Sean D. Plunkett, Frances H. Arnold, Molecularly imprinted polymers on silica: selective supports for high-performance ligand-exchange chromatography, Journal of Chromatography A, Volume 708, Issue 1, 1995, Pages 19-29, ISSN 0021-9673, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89004
Deposited By: Tony Diaz
Deposited On:22 Aug 2018 15:02
Last Modified:16 Nov 2021 00:31

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