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Association of antigens to ISCOMATRIX™ adjuvant using metal chelation leads to improved CTL responses

Malliaros, Jim and Quinn, Charles and Arnold, Frances H. and Pearse, Martin J. and Drane, Debbie P. and Stewart, Trina J. and Macfarlan, Roderick I. (2004) Association of antigens to ISCOMATRIX™ adjuvant using metal chelation leads to improved CTL responses. Vaccine, 22 (29-30). pp. 3968-3975. ISSN 0264-410X. https://resolver.caltech.edu/CaltechAUTHORS:20180822-090642552

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Abstract

The association of antigen with ISCOMATRIX™ adjuvant has been shown to be important for the optimal induction of cytotoxic T lymphocyte (CTL) responses. Here, we describe a simple broadly applicable method for associating recombinant proteins with hexa-histidine tags to ISCOMATRIX™ adjuvant utilising metal-affinity chelating interactions. The metal chelation binding step can be performed in a wide range of buffers, including commonly used denaturants such as urea, which makes it an ideal strategy for formulating proteins which are otherwise insoluble. Following association of protein with the chelating ISCOMATRIX™ adjuvant, the denaturant can be removed. Further, we show enhanced CTL responses with a protein-associated chelating ISCOMATRIX™ vaccine compared to a non-associated ISCOMATRIX™ vaccine.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.vaccine.2004.03.054DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2004 Elsevier Ltd. Received 22 December 2003, Revised 4 February 2004, Accepted 31 March 2004, Available online 27 April 2004. The authors thank H. Elmer and P. Schoofs for the provision of E6E7HH.We also thank R. Harris, J. Yanni, A. Coulter and S. Camuglia for assistance with formulation analysis, R. Hamilton who performed the electron microscopy and D. Airey who prepared graphs.
Subject Keywords:Chelating ISCOMATRIX™ adjuvant; ISCOMATRIX™ vaccine; HPV; CTL ISCOMATRIX™; ISCOM™ and ISCOPREP™ are trade marks of ISCOTEC AB a CSL Limited Company
Issue or Number:29-30
Record Number:CaltechAUTHORS:20180822-090642552
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180822-090642552
Official Citation:Jim Malliaros, Charles Quinn, Frances H. Arnold, Martin J. Pearse, Debbie P. Drane, Trina J. Stewart, Roderick I. Macfarlan, Association of antigens to ISCOMATRIX™ adjuvant using metal chelation leads to improved CTL responses, Vaccine, Volume 22, Issues 29–30, 2004, Pages 3968-3975, ISSN 0264-410X, https://doi.org/10.1016/j.vaccine.2004.03.054. (http://www.sciencedirect.com/science/article/pii/S0264410X04003214)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89014
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Aug 2018 16:20
Last Modified:03 Oct 2019 20:12

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