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Isolation of metaphase chromosomes from HeLa cells

Huberman, Joel A. and Attardi, Giuseppe (1966) Isolation of metaphase chromosomes from HeLa cells. Journal of Cell Biology, 31 (1). pp. 95-105. ISSN 0021-9525. PMCID PMC2107040. doi:10.1083/jcb.31.1.95.

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The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.

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Additional Information:© 1966 by Rockefeller University Press. Received for publication 4 April 1966. This work was supported by United States Public Health Service grants GM-11726 and 5-F1-GM-21,622. The authors gratefully acknowledge the help of Mr. John Elberfeld in part of this work and the valuable technical assistance of Mrs. Benneta Keeley and Mrs. LaVerne Wenzel.
Funding AgencyGrant Number
NIH Postdoctoral Fellowship5-F1-GM-21,622
Issue or Number:1
PubMed Central ID:PMC2107040
Record Number:CaltechAUTHORS:HUBjcb66
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8903
Deposited By: Archive Administrator
Deposited On:25 Sep 2007
Last Modified:08 Nov 2021 20:54

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