Huberman, Joel A. and Attardi, Giuseppe (1966) Isolation of metaphase chromosomes from HeLa cells. Journal of Cell Biology, 31 (1). pp. 95-105. ISSN 0021-9525. PMCID PMC2107040. doi:10.1083/jcb.31.1.95. https://resolver.caltech.edu/CaltechAUTHORS:HUBjcb66
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Abstract
The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.
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| Additional Information: | © 1966 by Rockefeller University Press. Received for publication 4 April 1966. This work was supported by United States Public Health Service grants GM-11726 and 5-F1-GM-21,622. The authors gratefully acknowledge the help of Mr. John Elberfeld in part of this work and the valuable technical assistance of Mrs. Benneta Keeley and Mrs. LaVerne Wenzel. | |||||||||
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| Issue or Number: | 1 | |||||||||
| PubMed Central ID: | PMC2107040 | |||||||||
| DOI: | 10.1083/jcb.31.1.95 | |||||||||
| Record Number: | CaltechAUTHORS:HUBjcb66 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:HUBjcb66 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 8903 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Archive Administrator | |||||||||
| Deposited On: | 25 Sep 2007 | |||||||||
| Last Modified: | 08 Nov 2021 20:54 |
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