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Noncontiguous SCHEMA Protein Recombination

Smith, Matthew A. and Arnold, Frances H. (2014) Noncontiguous SCHEMA Protein Recombination. In: Directed Evolution Library Creation: Methods and Protocols. Methods in Molecular Biology. No.1179. Humana Press , New York, NY, pp. 345-352. ISBN 9781493910526. http://resolver.caltech.edu/CaltechAUTHORS:20180822-140726128

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Abstract

SCHEMA is a method of designing protein recombination libraries that contain a large fraction of functional proteins with a high degree of mutational diversity. In the previous chapter, we illustrated the method for designing libraries by swapping contiguous sequence elements. Here, we introduce the NCR (“noncontiguous recombination”) algorithm to identify optimal designs for swapping elements that are contiguous in the 3-D structure but not necessarily in the primary sequence. To exemplify the method, NCR is used to recombine three fungal cellobiohydrolases (CBH1s) to produce a library containing more than 500,000 novel chimeric sequences.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/978-1-4939-1053-3_23DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© Springer Science+Business Media New York 2014. The authors acknowledge funding from the Institute for Collaborative Biotechnologies through grant W911NF-09-D-0001 from the US Army Research Office and the National Central University, Taiwan, through a Cooperative Agreement for Energy Research Collaboration. M.A.S. is supported by a Resnick Sustainability Institute fellowship.
Group:Resnick Sustainability Institute
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-D-0001
National Central University (Taiwan)UNSPECIFIED
Resnick Sustainability InstituteUNSPECIFIED
Subject Keywords:Protein engineering; Homologous recombination; SCHEMA; Noncontiguous recombination; NCR; Chimeragenesis
Record Number:CaltechAUTHORS:20180822-140726128
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180822-140726128
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89037
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Aug 2018 22:11
Last Modified:22 Aug 2018 22:11

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