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Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes

Pack, Daniel W. and Arnold, Frances H. (1997) Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes. Chemistry and Physics of Lipids, 86 (2). pp. 135-152. ISSN 0009-3084. https://resolver.caltech.edu/CaltechAUTHORS:20180822-140726582

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Abstract

Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating lipids. The lipids utilize the metal chelator iminodiacetate (IDA) as the hydrophilic headgroup, allowing display of divalent transition metal ions on the aqueous side of the membrane. Changes in surface pressure-molecular area isotherms were used to observe metal binding, and an association constant for Cu^(2+) binding to the IDA lipids of 10^(7–8) M^(−1) was estimated. The ability to control binding site density is important for many applications. The IDA lipid was found to be miscible with both distearoylphosphocholine (DSPC) and 1-stearoyl-2-oleoyl-phosphocholine (SOPC) at most compositions and surface pressures.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0009-3084(97)02662-5DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1997 Elsevier. Received 26 September 1996, Revised 9 January 1997, Accepted 9 January 1997.
Subject Keywords:Protein immobilization; Functionalized surfaces; Iminodiacetate-copper; Fluorescence microscopy; Molecular recognition
Issue or Number:2
Record Number:CaltechAUTHORS:20180822-140726582
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180822-140726582
Official Citation:Daniel W. Pack, Frances H. Arnold, Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes, Chemistry and Physics of Lipids, Volume 86, Issue 2, 1997, Pages 135-152, ISSN 0009-3084, https://doi.org/10.1016/S0009-3084(97)02662-5. (http://www.sciencedirect.com/science/article/pii/S0009308497026625)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89042
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Aug 2018 22:39
Last Modified:03 Oct 2019 20:12

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