CaltechAUTHORS
  A Caltech Library Service

Characterization of His-X_3-His sites in α-helices of synthetic metal-binding bovine somatotropin

Suh, Sung-Sup and Haymore, Barry L. and Arnold, Frances H. (1991) Characterization of His-X_3-His sites in α-helices of synthetic metal-binding bovine somatotropin. Protein Engineering, Design and Selection, 4 (3). pp. 301-305. ISSN 1741-0126. https://resolver.caltech.edu/CaltechAUTHORS:20180822-140907750

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20180822-140907750

Abstract

Variants of bovine somatotropin have been engineered to contain synthetic metal-binding sites consisting of two solvent-exposed histidines separated by a single turn of an α-helix (His-X_3-His variants). The affinities of these proteins for Cu(II) were characterized by measuring their partitioning coefficients in an aqueous two-phase polymer system. The partition coefficients were used to generate binding constants for formation of a complex between the engineered metal-binding site and Cu(II) chelated to an iminodiacetic acid derivative of polyethylene glycol. For three His-X_3-His variants described here, these constants range from 2×104 to 1.66×610^6 M^(-1). The metal affinity of a His-X_3-His site depends on the rigidity of the helix into which the site is engineered. The affinities of the His-X_3-His sites for Cu(II) are large enough to dramatically increase not only the partitioning of these proteins in aqueous two-phase systems, but also their retention times on a metal-affinity chromatography column. Both these features can greatly facilitate the purification of engineered proteins. Criteria for choosing positions for incorporating metal-binding sites are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/protein/4.3.301DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Alternate Title:Characterization of His-X3-His sites in α-helices of synthetic metal-binding bovine somatotropin
Additional Information:© 1991 Oxford University Press.
Issue or Number:3
Record Number:CaltechAUTHORS:20180822-140907750
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180822-140907750
Official Citation:Sung-Sup Suh, Barry L. Haymore, Frances H. Arnold; Characterization of His-X3-His sites in α-helices of synthetic metal-binding bovine somatotropin, Protein Engineering, Design and Selection, Volume 4, Issue 3, 1 February 1991, Pages 301–305, https://doi.org/10.1093/protein/4.3.301
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89056
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Aug 2018 22:09
Last Modified:03 Oct 2019 20:12

Repository Staff Only: item control page