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Metal-Affinity Separations: A New Dimension in Protein Processing

Arnold, Frances H. (1991) Metal-Affinity Separations: A New Dimension in Protein Processing. Bio/Technology, 9 (2). pp. 151-156. ISSN 0733-222X. https://resolver.caltech.edu/CaltechAUTHORS:20180822-141010067

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Abstract

Rapid growth in the preparative and high-resolution analytical applications of metal-affinity chromatography demonstrate the appeal of metal recognition as a basis for protein separations. Stable, inexpensive chelated metals effectively mimic bio-specific interactions, providing selective ligands for protein binding. This article reviews recent progress in understanding the mechanisms of metal-protein recognition that underlie metal-affinity separations. Also discussed are schemes for integrating metal-affinity purifications into the expression and bioprocessing of re-combinant proteins. Promising future developments include new metal-affinity processes for analytical and preparative-scale separations and a range of techniques for enhancing the selectivity of metal-affinity separations.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/nbt0291-151DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1991 Springer Nature Limited.
Issue or Number:2
Record Number:CaltechAUTHORS:20180822-141010067
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180822-141010067
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89057
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Aug 2018 21:36
Last Modified:03 Oct 2019 20:12

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