Todd, Robert J. and Van Dam, Mariana E. and Casimiro, Danilo and Haymore, Barry L. and Arnold, Frances H. (1991) Cu(II)-Binding properties of a cytochrome c with a synthetic metal-binding site: His-X_3-His in an α-helix. Proteins, 10 (2). pp. 156-161. ISSN 0887-3585. doi:10.1002/prot.340100209. https://resolver.caltech.edu/CaltechAUTHORS:20180822-141010187
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Abstract
A metal‐binding site consisting of two histidines positioned His‐X_3‐His in an α‐helix has been engineered into the surface of Saccharomyces cerevisiae iso‐1‐cytochrome c. The synthetic metal‐binding cytochrome c retains its biological activity in vivo. Its ability to bind chelated Cu(II) has been characterized by partitioning in aqueous two‐phase polymer systems containing a polymer‐metal complex, Cu(II)IDA‐PEG, and by metal‐affinity chromatography. The stability constant for the complex formed between Cu(II)IDA‐PEG and the cytochrome c His‐X3‐His site is 5.3 × 104^ M^(−1), which corresponds to a chelate effect that contributes 1.5 kcal mol^(−1) to the binding energy. Incorporation of the His‐X_3‐His site yields a synthetic metal‐binding protein whose metal affinity is sensitive to environmental conditions that alter helix structure or flexibility.
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Alternate Title: | Cu(II)-Binding properties of a cytochrome c with a synthetic metal-binding site: His-X3-His in an α-helix | ||||||
Additional Information: | © 1991 Wiley‐Liss. Manuscript received: 01 November 1990. Manuscript accepted: 18 January 1991. | ||||||
Subject Keywords: | synthetic metalloproteins; protein engineering; iso‐1‐cytochrome c; metal binding | ||||||
Issue or Number: | 2 | ||||||
DOI: | 10.1002/prot.340100209 | ||||||
Record Number: | CaltechAUTHORS:20180822-141010187 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180822-141010187 | ||||||
Official Citation: | Todd, R. J., Van Dam, M. E., Casimiro, D. , Haymore, B. L. and Arnold, F. H. (1991), Cu(II)‐Binding properties of a cytochrome c with a synthetic metal‐binding site: His‐X3‐His in an α‐helix. Proteins, 10: 156-161. doi:10.1002/prot.340100209 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 89058 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | George Porter | ||||||
Deposited On: | 22 Aug 2018 21:38 | ||||||
Last Modified: | 16 Nov 2021 00:31 |
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