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A Self-Sufficient Peroxide-Driven Hydroxylation Biocatalyst

Cirino, Patrick C. and Arnold, Frances H. (2003) A Self-Sufficient Peroxide-Driven Hydroxylation Biocatalyst. Angewandte Chemie International Edition, 42 (28). pp. 3299-3301. ISSN 1433-7851. doi:10.1002/anie.200351434.

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Directed evolution of the heme domain of cytochrome P450 BM‐3 has resulted in a versatile, highly active peroxide‐driven hydroxylation catalyst (see picture) that requires neither NADPH nor reductase and functions in a cell‐free reaction system. This simplified, “biomimetic” catalyst is amenable to further optimization, for example, to improve stability or alter its substrate range.

Item Type:Article
Related URLs:
URLURL TypeDescription
Cirino, Patrick C.0000-0002-7245-6205
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2003 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. Received: March 18, 2003. This work was supported by the Biotechnology Research and Development Corporation (Peoria, IL) and an NSF Graduate Research Fellowship awarded to P.C.C. The authors thank Ulrich Schwaneberg for guidance and Denis Shcherbakov for laboratory assistance.
Funding AgencyGrant Number
Biotechnology Research and Development CorporationUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
Subject Keywords:biocatalysis; cytochrome P450; enzymes; oxidation; peroxygenase
Issue or Number:28
Record Number:CaltechAUTHORS:20180822-145522004
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Official Citation:Cirino, P. C. and Arnold, F. H. (2003), A Self‐Sufficient Peroxide‐Driven Hydroxylation Biocatalyst. Angewandte Chemie International Edition, 42: 3299-3301. doi:10.1002/anie.200351434
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89063
Deposited By: Tony Diaz
Deposited On:22 Aug 2018 22:00
Last Modified:16 Nov 2021 00:31

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