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Directed evolution of enzyme catalysts

Kuchner, Olga and Arnold, Frances H. (1997) Directed evolution of enzyme catalysts. Trends in Biotechnology, 15 (12). pp. 523-530. ISSN 0167-7799. doi:10.1016/S0167-7799(97)01138-4.

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Directed enzyme evolution has emerged in the past few years as a powerful alternative to rational approaches for engineering biocatalysts. Prerequisites for successful directed evolution are functional expression in a suitable microbial host, a rapid screen for the desired feature(s) and a well-thought-out working strategy for navigating protein landscapes. The rapidly growing body of literature on enzyme evolution in vitro includes techniques for creating and searching combinatorial enzyme libraries, as well as several successful examples of different evolutionary strategies being used.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1997 Published by Elsevier Ltd. Available online 3 February 1998. Support from the Army Research Office, the Office of Naval Research and the Department of Energy's program in Biological and Chemical Technologies Research is acknowledged. O. Kuchner is the recipient of an NIH predoctoral training fellowship from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01. The authors thank Daniel Song for helpful discussions.
Funding AgencyGrant Number
Army Research Office (ARO)UNSPECIFIED
Office of Naval Research (ONR)UNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
NIH Predoctoral Fellowship1 T32 GM 08346-01
Issue or Number:12
Record Number:CaltechAUTHORS:20180822-161250434
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Official Citation:Olga Kuchner, Frances H. Arnold, Directed evolution of enzyme catalysts, Trends in Biotechnology, Volume 15, Issue 12, 1997, Pages 523-530, ISSN 0167-7799, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89068
Deposited By: Tony Diaz
Deposited On:23 Aug 2018 13:59
Last Modified:16 Nov 2021 00:32

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