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Evolving strategies for enzyme engineering

Bloom, Jesse D. and Meyer, Michelle M. and Meinhold, Peter and Otey, Christopher R. and MacMillan, Derek and Arnold, Frances H. (2005) Evolving strategies for enzyme engineering. Current Opinion in Structural Biology, 15 (4). pp. 447-452. ISSN 0959-440X. http://resolver.caltech.edu/CaltechAUTHORS:20180823-073228047

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Abstract

Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties. Strategies that target mutagenesis to particular regions of a protein or use recombination to introduce large sequence changes can complement full-gene random mutagenesis and pave the way to achieving ever more ambitious enzyme engineering goals.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.sbi.2005.06.004DOIArticle
ORCID:
AuthorORCID
Bloom, Jesse D.0000-0003-1267-3408
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2005 Elsevier Ltd. Available online 11 July 2005. JDB and MMM are supported by Howard Hughes Medical Institute pre-doctoral fellowships, DM is supported by the Royal Society. FHA acknowledges support from the US National Science Foundation, the Army Research Office (DAAD19-03-D-0004) and the National Institutes of Health (grant R01 GM068664-01).
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Royal SocietyUNSPECIFIED
NSFUNSPECIFIED
Army Research Office (ARO)DAAD19-03-D-0004
NIHR01 GM068664-01
Record Number:CaltechAUTHORS:20180823-073228047
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180823-073228047
Official Citation:Jesse D Bloom, Michelle M Meyer, Peter Meinhold, Christopher R Otey, Derek MacMillan, Frances H Arnold, Evolving strategies for enzyme engineering, Current Opinion in Structural Biology, Volume 15, Issue 4, 2005, Pages 447-452, ISSN 0959-440X, https://doi.org/10.1016/j.sbi.2005.06.004. (http://www.sciencedirect.com/science/article/pii/S0959440X0500120X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89071
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Aug 2018 16:48
Last Modified:23 Aug 2018 16:48

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