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Analytical affinity chromatography: II. Rate theory and the measurement of biological binding kinetics

Arnold, F. H. and Blanch, H. W. (1986) Analytical affinity chromatography: II. Rate theory and the measurement of biological binding kinetics. Journal of Chromatography A, 355 . pp. 13-27. ISSN 0021-9673. http://resolver.caltech.edu/CaltechAUTHORS:20180823-083642906

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Abstract

Affinity chromatography can be used to measure equilibrium constants and kinetics of biological interactions. The local-equilibrium theory presented in the preceding paper is extended to include mass transfer and kinetic effects. Solutions for both zonal and frontal elution are presented. For highly nonlinear isotherms, the frontal elution method is preferred. Experiments with bovine serum albumin binding to immobilized Reactive Blue show that the binding kinetics inside the porous gel are several orders of magnitude slower than typical biological binding reactions in solution. The temperature dependence of the kinetic constants indicate that the binding may still be diffusion-controlled.


Item Type:Article
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https://doi.org/10.1016/S0021-9673(01)97300-5DOIArticle
ORCID:
AuthorORCID
Arnold, F. H.0000-0002-4027-364X
Additional Information:© 1986 Published by Elsevier B.V. Received 1 October 1985. The authors wish to thank Jack Hwang for his helpful contributions to this work. This research was funded by the Center for Biotechnology Research, San Francisco, CA.
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Center for Biotechnology ResearchUNSPECIFIED
Record Number:CaltechAUTHORS:20180823-083642906
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180823-083642906
Official Citation:F.H. Arnold, H.W. Blanch, Analytical affinity chromatography: II. Rate theory and the measurement of biological binding kinetics, Journal of Chromatography A, Volume 355, 1986, Pages 13-27, ISSN 0021-9673, https://doi.org/10.1016/S0021-9673(01)97300-5. (http://www.sciencedirect.com/science/article/pii/S0021967301973005)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89080
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Aug 2018 16:55
Last Modified:23 Aug 2018 16:55

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