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Metal affinity partitioning

Chung, Bong H. and Bailey, Darwin and Arnold, Frances H. (1994) Metal affinity partitioning. In: Aqueous Two-Phase Systems. Methods in Enzymology. No.228. Academic Press , San Diego, CA, pp. 167-179. ISBN 9780121821296.

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The partitioning of biological materials in aqueous two-phase systems, for example, poly(ethylene glycol) (PEG)-dextran (Dx) systems, can be altered in a reproducible and predictable fashion using affinity ligands attached to one of the phase-forming polymers. Metal ion complexes such as copper(II) iminodiacetate [Cu(II)IDA] have several very attractive features as affinity ligands: they are stable under a wide range of solvent conditions and temperatures and are therefore easy to recycle; they can be incorporated into chromatographic supports with very high loading capacities; their interactions with target molecules are reasonably specific and are reversible under mild conditions; and they are inexpensive.

Item Type:Book Section
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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1994 by Academic Press, Inc. This research is supported by the National Science Foundation, Grant No. EET-8807351, and a Presidential Young Investigator Award (F.H.A.). F.H.A. gratefully acknowledges a fellowship from the David and Lucile Packard Foundation. D.B. is the recipient of a fellowship from the Irvine Foundation.
Funding AgencyGrant Number
David and Lucile Packard FoundationUNSPECIFIED
James Irvine FoundationUNSPECIFIED
Series Name:Methods in Enzymology
Issue or Number:228
Record Number:CaltechAUTHORS:20180823-084045820
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Official Citation:Bong H. Chung, Darwin Bailey, Frances H. Arnold, [15] Metal affinity partitioning, Methods in Enzymology, Academic Press, Volume 228, 1994, Pages 167-179, ISSN 0076-6879, ISBN 9780121821296, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89081
Deposited By: Tony Diaz
Deposited On:23 Aug 2018 17:09
Last Modified:03 Oct 2019 20:13

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