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The temkin isotherm describes heterogeneous protein adsorption

Johnson, Robert D. and Arnold, Frances H. (1995) The temkin isotherm describes heterogeneous protein adsorption. Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1247 (2). pp. 293-297. ISSN 0167-4838. doi:10.1016/0167-4838(95)00006-G.

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Here we examine how heterogeneous protein adsorption arises from multivalent interactions with a seemingly homogeneous functional surface. During adsorption, some arrangement of functional groups on the protein (e.g., charged or hydrophobic amino-acid residues or specific ligand binding sites) interacts with complementary sites distributed on the adsorbent surface. The protein will show the highest affinity for the surface arrangements which best match its own distribution of functional sites, resulting in a distribution of binding energies. To support this interpretation, we show that changing the density of affinity ligands on a surface (immobilized metal ions) is equivalent to changing the number of target groups on a protein (surface histidines). We also report that reversible protein adsorption obeys the Temkin isotherm and propose that model as a practical framework for describing the behavior of proteins adsorbing via multivalent interactions onto surfaces densely derivatized with a random distribution of binding functionalities. This result has important implications for the design of separations materials and the interpretation of biological recognition phenomena.

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Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 1995 Published by Elsevier B.V. Received 6 December 1994, Accepted 29 December 1994. This research is supported by the U.S. Office of Naval Research and the National Science Foundation. F.H.A. acknowledges an NSF PYI award and a fellowship from the David and Lucile Packard Foundation. R.D.J. is supported by a predoctoral training fellowship from the U.S. National Institute of General Medical Sciences, Pharmacology Sciences Program.
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Subject Keywords:Biological recognition; Heterogeneous binding; Histidine; Metal affinity chromatography; Copper iminodiacetic acid
Issue or Number:2
Record Number:CaltechAUTHORS:20180823-144019749
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Official Citation:Robert D. Johnson, Frances H. Arnold, The temkin isotherm describes heterogeneous protein adsorption, Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Volume 1247, Issue 2, 1995, Pages 293-297, ISSN 0167-4838, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89099
Deposited By: Tony Diaz
Deposited On:23 Aug 2018 21:46
Last Modified:16 Nov 2021 00:32

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