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Analytical affinity chromatography: I. Local equilibrium theory and the measurement of association and inhibition constants

Arnold, F. H. and Schofield, S. A. and Blanch, H. W. (1986) Analytical affinity chromatography: I. Local equilibrium theory and the measurement of association and inhibition constants. Journal of Chromatography A, 355 . pp. 1-12. ISSN 0021-9673. http://resolver.caltech.edu/CaltechAUTHORS:20180823-144311722

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Abstract

Affinity chromatography can be used as an analytical tool to measure the binding constants for biological interactions. Most chromatography theories are based on the assumption that the equilibrium isotherm is linear, a poor assumption for most biological interactions. This paper presents a local-equilibrium theory for nonlinear zonal and frontal elution chromatography. Results are compared to other commonly-used affinity chromatography elution equations. The effect of isotherm nonlinearity is shown to be relatively small, provided the product of the association constant and feed concentration is less than 1. The major effect of nonlinearity is zone spreading; this can be very important when measuring rate constants. The measurement of binding kinetics by analytical affinity chromatography is discussed in Part II.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0021-9673(01)97299-1DOIArticle
ORCID:
AuthorORCID
Arnold, F. H.0000-0002-4027-364X
Additional Information:© 1986 Published by Elsevier. Received 1 October 1985.
Record Number:CaltechAUTHORS:20180823-144311722
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180823-144311722
Official Citation:F.H. Arnold, S.A. Schofield, H.W. Blanch, Analytical affinity chromatography: I. Local equilibrium theory and the measurement of association and inhibition constants, Journal of Chromatography A, Volume 355, 1986, Pages 1-12, ISSN 0021-9673, https://doi.org/10.1016/S0021-9673(01)97299-1. (http://www.sciencedirect.com/science/article/pii/S0021967301972991)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89112
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Aug 2018 22:38
Last Modified:23 Aug 2018 22:38

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