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Designed evolution of enzymatic properties

Petrounia, Ioanna P. and Arnold, Frances H. (2000) Designed evolution of enzymatic properties. Current Opinion in Biotechnology, 11 (4). pp. 325-330. ISSN 0958-1669. https://resolver.caltech.edu/CaltechAUTHORS:20180823-155805952

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Abstract

By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis. Recent advances include the evolution of a novel catalytic activity using the α/β barrel scaffold, evolution of a cofactor-free monooxygenase, and the engineering of regulatable enzymes. New screening systems for enantioselectivity and protein solubility, and the continuing stream of new methods for creating enzyme libraries further extend evolution’s reach.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/s0958-1669(00)00107-5DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2000 Elsevier. Available online 2 August 2000.
Subject Keywords:Directed evolution; Biocatalysis; Cytochrome P450; Protein engineering; Random mutagenesis; In vitro recombinatin; DNA shuffling; High-throughput screening; Selection
Issue or Number:4
Record Number:CaltechAUTHORS:20180823-155805952
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180823-155805952
Official Citation:Ioanna P Petrounia, Frances H Arnold, Designed evolution of enzymatic properties, Current Opinion in Biotechnology, Volume 11, Issue 4, 2000, Pages 325-330, ISSN 0958-1669, https://doi.org/10.1016/S0958-1669(00)00107-5. (http://www.sciencedirect.com/science/article/pii/S0958166900001075)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89117
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:23 Aug 2018 23:05
Last Modified:03 Oct 2019 20:13

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