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Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3

Meinhold, Peter and Peters, Matthew W. and Chen, Michael M. Y. and Takahashi, Katsuyuki and Arnold, Frances H. (2005) Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3. ChemBioChem, 6 (10). pp. 1765-1768. ISSN 1439-4227. doi:10.1002/cbic.200500261. https://resolver.caltech.edu/CaltechAUTHORS:20180824-074157610

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Abstract

Picking on someone smaller. Cytochromes P450 catalyze the hydroxylation of thousands of substrates, including alkanes. No naturally occurring P450, however, is known to oxidize the smallest alkanes, ethane and methane. Here we report the direct and selective oxidation of ethane to ethanol using dioxygen, catalyzed by a cytochrome P450 BM‐3 variant engineered for high activity towards small alkanes (see scheme). Achieving P450‐catalyzed oxidation of ethane is a key step in the pathway to P450‐catalyzed methane oxidation and opens new opportunities for the bioconversion of natural gas to fuels and chemicals.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/cbic.200500261DOIArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. Received: May 25, 2005; Published online on August 4, 2005. The authors thank Nathan Dalleska for assistance with the gas chromatography. This work is supported by the National Science Foundation (BES 9981770).
Funders:
Funding AgencyGrant Number
NSFBES-9981770
Subject Keywords:alkanes; cytochromes; directed evolution; enzyme catalysis; hydroxylation
Issue or Number:10
DOI:10.1002/cbic.200500261
Record Number:CaltechAUTHORS:20180824-074157610
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180824-074157610
Official Citation:Meinhold, P. , Peters, M. W., Chen, M. M., Takahashi, K. and Arnold, F. H. (2005), Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3. ChemBioChem, 6: 1765-1768. doi:10.1002/cbic.200500261
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89119
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Aug 2018 14:47
Last Modified:16 Nov 2021 00:32

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