Meinhold, Peter and Peters, Matthew W. and Chen, Michael M. Y. and Takahashi, Katsuyuki and Arnold, Frances H. (2005) Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3. ChemBioChem, 6 (10). pp. 1765-1768. ISSN 1439-4227. doi:10.1002/cbic.200500261. https://resolver.caltech.edu/CaltechAUTHORS:20180824-074157610
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Abstract
Picking on someone smaller. Cytochromes P450 catalyze the hydroxylation of thousands of substrates, including alkanes. No naturally occurring P450, however, is known to oxidize the smallest alkanes, ethane and methane. Here we report the direct and selective oxidation of ethane to ethanol using dioxygen, catalyzed by a cytochrome P450 BM‐3 variant engineered for high activity towards small alkanes (see scheme). Achieving P450‐catalyzed oxidation of ethane is a key step in the pathway to P450‐catalyzed methane oxidation and opens new opportunities for the bioconversion of natural gas to fuels and chemicals.
| Item Type: | Article | ||||||
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| Additional Information: | © 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim. Received: May 25, 2005; Published online on August 4, 2005. The authors thank Nathan Dalleska for assistance with the gas chromatography. This work is supported by the National Science Foundation (BES 9981770). | ||||||
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| Subject Keywords: | alkanes; cytochromes; directed evolution; enzyme catalysis; hydroxylation | ||||||
| Issue or Number: | 10 | ||||||
| DOI: | 10.1002/cbic.200500261 | ||||||
| Record Number: | CaltechAUTHORS:20180824-074157610 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20180824-074157610 | ||||||
| Official Citation: | Meinhold, P. , Peters, M. W., Chen, M. M., Takahashi, K. and Arnold, F. H. (2005), Direct Conversion of Ethane to Ethanol by Engineered Cytochrome P450 BM3. ChemBioChem, 6: 1765-1768. doi:10.1002/cbic.200500261 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 89119 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Tony Diaz | ||||||
| Deposited On: | 24 Aug 2018 14:47 | ||||||
| Last Modified: | 16 Nov 2021 00:32 |
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