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Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases

Alcalde, Miguel and Bulter, Thomas and Arnold, Frances H. (2002) Colorimetric Assays for Biodegradation of Polycyclic Aromatic Hydrocarbons by Fungal Laccases. Journal of Biomolecular Screening, 7 (6). pp. 547-553. ISSN 1087-0571. doi:10.1177/1087057102238629. https://resolver.caltech.edu/CaltechAUTHORS:20180824-095915317

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Abstract

Polycyclic aromatic hydrocarbons (PAHs) are highly toxic organic pollutants widely distributed in terrestrial and aquatic environments. In the present work, 2 colorimetric assays for laccase-catalyzed degradation of PAHs were developed based on studies of the oxidation of 12 aromatic hydrocarbons by fungal laccases from Trametes versicolor and Myceliophthora thermophila. Using a sodium borohydride water-soluble solution, the authors could reduce the single product of laccase-catalyzed anthracene biooxidation into the orange-colored 9,10-anthrahydroquinone, which is quantifiable spectrophotometrically. An assay using polymeric dye (Poly R-478) as a surrogate substrate for lignin degradation by laccase in the presence of mediator is also presented. The decolorization of Poly R-478 was correlated to the oxidation of PAHs mediated by laccases. This demonstrates that a ligninolytic indicator such as Poly R-478 can be used to screen for PAH-degrading laccases; it will also be useful in screening mutant libraries in directed evolution experiments. Poly R-478 is stable and readily soluble. It has a high extinction coefficient and low toxicity toward white rot fungi, yeast, and bacteria, which allow its application in a solid-phase assay format.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1177/1087057102238629DOIArticle
ORCID:
AuthorORCID
Alcalde, Miguel0000-0001-6780-7616
Arnold, Frances H.0000-0002-4027-364X
Additional Information:© 2002 The Society for Biomolecular Screening. This work was supported by the U.S. Office of Naval Research. We thank the Ministerio de Educacion y Cultura of Spain (MA) and Deutsche Forschungsgemeinschaft (TB) for fellowships.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
Ministerio de Educacion y Cultura (MEC)UNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Issue or Number:6
DOI:10.1177/1087057102238629
Record Number:CaltechAUTHORS:20180824-095915317
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180824-095915317
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89129
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Aug 2018 17:13
Last Modified:16 Nov 2021 00:32

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