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Nitric Oxide Modulates Endonuclease III Redox Activity by a 800 mV Negative Shift upon [Fe_4S_4] Cluster Nitrosylation

Ekanger, Levi A. and Oyala, Paul H. and Moradian, Annie and Sweredoski, Michael J. and Barton, Jacqueline K. (2018) Nitric Oxide Modulates Endonuclease III Redox Activity by a 800 mV Negative Shift upon [Fe_4S_4] Cluster Nitrosylation. Journal of the American Chemical Society, 140 (37). pp. 11800-11810. ISSN 0002-7863. PMCID PMC6186442. doi:10.1021/jacs.8b07362. https://resolver.caltech.edu/CaltechAUTHORS:20180827-094622072

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Abstract

Here we characterize the [Fe_4S_4] cluster nitrosylation of a DNA repair enzyme, endonuclease III (EndoIII), using DNA-modified gold electrochemistry and protein film voltammetry, electrophoretic mobility shift assays, mass spectrometry of whole and trypsin-digested protein, and a variety of spectroscopies. Exposure of EndoIII to nitric oxide under anaerobic conditions transforms the [Fe_4S_4] cluster into a dinitrosyl iron complex, [(Cys)_2Fe(NO)_2]−, and Roussin’s red ester, [(μ-Cys)_2Fe_2(NO)_4], in a 1:1 ratio with an average retention of 3.05 ± 0.01 Fe per nitrosylated cluster. The formation of the dinitrosyl iron complex is consistent with previous reports, but the Roussin’s red ester is an unreported product of EndoIII nitrosylation. Hyperfine sublevel correlation (HYSCORE) pulse EPR spectroscopy detects two distinct classes of NO with ^(14)N hyperfine couplings consistent with the dinitrosyl iron complex and reduced Roussin’s red ester. Whole-protein mass spectrometry of EndoIII nitrosylated with ^(14)NO and ^(15)NO support the assignment of a protein-bound [(μ-Cys)_2Fe_2(NO))_4] Roussin’s red ester. The [Fe_4S_4]^(2+/3+) redox couple of DNA-bound EndoIII is observable using DNA-modified gold electrochemistry, but nitrosylated EndoIII does not display observable redox activity using DNA electrochemistry on gold despite having a similar DNA-binding affinity as the native protein. However, direct electrochemistry of protein films on graphite reveals the reduction potential of native and nitrosylated EndoIII to be 127 ± 6 and −674 ± 8 mV vs NHE, respectively, corresponding to a shift of approximately −800 mV with cluster nitrosylation. Collectively, these data demonstrate that DNA-bound redox activity, and by extension DNA-mediated charge transport, is modulated by [Fe_4S_4] cluster nitrosylation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1021/jacs.8b07362DOIArticle
https://pubs.acs.org/doi/suppl/10.1021/jacs.8b07362PublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6186442PubMed CentralArticle
ORCID:
AuthorORCID
Ekanger, Levi A.0000-0001-8131-1641
Oyala, Paul H.0000-0002-8761-4667
Moradian, Annie0000-0002-0407-2031
Sweredoski, Michael J.0000-0003-0878-3831
Barton, Jacqueline K.0000-0001-9883-1600
Alternate Title:Nitric Oxide Modulates Endonuclease III Redox Activity by a 800 mV Negative Shift upon [Fe4S4] Cluster Nitrosylation
Additional Information:© 2018 American Chemical Society. Received: July 12, 2018; Published: August 26, 2018. This work was supported by GM126904 from the National Institutes of Health (to J.K.B.). The Caltech EPR facility is supported by the National Science Foundation (NSF-1531940) and the Dow Next Generation Educator Fund. The Proteome Exploration Laboratory is supported by the Beckman Institute and the National Institutes of Health (1S10OD02001301). This research benefited from the use of the Autoflex MALDI TOF mass spectrometer in the Caltech CCE Multiuser Mass Spectrometry Laboratory, acquired with funds from the Dow Corporation. The authors thank Andy Zhou for assistance with protein overexpression and purification. The authors declare no competing financial interest.
Funders:
Funding AgencyGrant Number
NIHGM126904
NSFCHE-1531940
Dow Next Generation Educator FundUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
NIH1S10OD02001301
Dow Chemical CompanyUNSPECIFIED
Issue or Number:37
PubMed Central ID:PMC6186442
DOI:10.1021/jacs.8b07362
Record Number:CaltechAUTHORS:20180827-094622072
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180827-094622072
Official Citation:Nitric Oxide Modulates Endonuclease III Redox Activity by a 800 mV Negative Shift upon [Fe4S4] Cluster Nitrosylation. Levi A. Ekanger, Paul H. Oyala, Annie Moradian, Michael J. Sweredoski, and Jacqueline K. Barton. Journal of the American Chemical Society 2018 140 (37), 11800-11810. DOI: 10.1021/jacs.8b07362
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89164
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:27 Aug 2018 22:16
Last Modified:16 Nov 2021 00:33

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