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Photoacoustic microscopy enables multilayered histological imaging of human breast cancer without staining

Wong, Terence T. W. and Zhang, Ruiying and Hai, Pengfei and Aft, Rebecca L. and Novack, Deborah V. and Wang, Lihong V. (2018) Photoacoustic microscopy enables multilayered histological imaging of human breast cancer without staining. In: Photons Plus Ultrasound: Imaging and Sensing 2018. Proceedings of SPIE. No.10494. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 1049443. ISBN 9781510614734. https://resolver.caltech.edu/CaltechAUTHORS:20180905-075329068

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Abstract

In 2016, an estimated ~250,000 new cases of invasive and non-invasive breast cancer were diagnosed in US women. About 60–75% of these cases were treated with breast conserving surgery (BCS) as the initial therapy. To reduce the local recurrence rate, the goal of BCS is to excise the tumor with a rim of normal surrounding tissue, so that no cancer cells remain at the cut margin, while preserving as much normal breast tissue as possible. Therefore, patients with remaining cancer cells at the cut margin commonly require a second surgical procedure to obtain clear margins. Different approaches have been used to decrease the positive margin rate to avoid re-excision. However, these techniques are variously ineffective in reducing the re-operative rate, difficult to master by surgeons, or time-consuming for large specimens. Thus, 20-60% of patients undergoing BCS still require second surgeries due to positive surgical margins. The ideal tool for margin assessment would provide the same information as histological analysis, without the need for processing specimens. To achieve this goal, we have developed and refined label-free photoacoustic microscopy (PAM) for breast specimens. Exploiting the intrinsic optical contrast of tissue, ultraviolet (UV) laser illumination can highlight cell nuclei, thus providing the same contrast as hematoxylin labeling used in conventional histology and measuring features related to the histological landscape without the need for labels. We demonstrate that our UV-PAM system can provide label-free, high-resolution, and histology-like imaging of fixed, unprocessed breast tissue.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2291008DOIArticle
ORCID:
AuthorORCID
Wong, Terence T. W.0000-0001-6399-758X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Society of Photo-Optical Instrumentation Engineers.
Subject Keywords:Cancer, translational research, photoacoustic imaging, microscopy, label-free, histology
Series Name:Proceedings of SPIE
Issue or Number:10494
Record Number:CaltechAUTHORS:20180905-075329068
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180905-075329068
Official Citation:Terence T. W. Wong, Terence T. W. Wong, Ruiying Zhang, Ruiying Zhang, Pengfei Hai, Pengfei Hai, Rebecca L. Aft, Rebecca L. Aft, Deborah V. Novack, Deborah V. Novack, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic microscopy enables multilayered histological imaging of human breast cancer without staining", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 1049443 (19 February 2018); doi: 10.1117/12.2291008; https://doi.org/10.1117/12.2291008
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89374
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Sep 2018 16:12
Last Modified:03 Oct 2019 20:15

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