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Label-free optical-resolution photoacoustic endomicroscopy in vivo

Yang, Joon-Mo and Li, Chiye and Chen, Ruimin and Rao, Bin and Yao, Junjie and Yeh, Cheng-Hung and Danielli, Amos and Maslov, Konstantin and Zhou, Qifa and Shung, K. Kirk and Wang, Lihong V. (2015) Label-free optical-resolution photoacoustic endomicroscopy in vivo. In: Photons Plus Ultrasound: Imaging and Sensing 2015. Proceedings of SPIE. No.9323. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 932332. ISBN 9781628414134.

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Intravital microscopy techniques have become increasingly important in biomedical research because they can provide unique microscopic views of various biological or disease developmental processes in situ. Here we present an optical-resolution photoacoustic endomicroscopy (OR-PAEM) system that visualizes internal organs with a much finer resolution than conventional acoustic-resolution photoacoustic endoscopy systems. By combining gradient index (GRIN) lens-based optical focusing and ultrasonic ring transducer-based acoustic focusing, we achieved a transverse resolution as fine as ~10 μm at an optical working distance of 6.5 mm. The OR-PAEM system’s high-resolution intravital imaging capability is demonstrated through animal experiments.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Chen, Ruimin0000-0002-5338-359X
Rao, Bin0000-0001-6494-3110
Maslov, Konstantin0000-0003-3408-8840
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2015 Society of Photo-Optical Instrumentation Engineers. We thank Professor James Ballard for his attentive reading of the manuscript. This work was sponsored in part by National Institutes of Health grants R01 CA157277, DP1 EB016986 (NIH Director’s Pioneer Award), P41-EB002182, and R01 CA186567 (NIH Director’s Transformative Research Award). L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. K.M. has a financial interest in Microphotoacoustics, Inc.
Funding AgencyGrant Number
NIHR01 CA157277
NIHDP1 EB016986
NIHR01 CA186567
Subject Keywords:Photoacoustic endoscopy, optical-resolution, photoacoustic endomicroscopy, photoacoustic microscopy, intravital microscopy, label-free imaging, in vivo imaging, rat colorectum
Series Name:Proceedings of SPIE
Issue or Number:9323
Record Number:CaltechAUTHORS:20180905-143111348
Persistent URL:
Official Citation:Joon-Mo Yang, Joon-Mo Yang, Chiye Li, Chiye Li, Ruimin Chen, Ruimin Chen, Bin Rao, Bin Rao, Junjie Yao, Junjie Yao, Cheng-Hung Yeh, Cheng-Hung Yeh, Amos Danielli, Amos Danielli, Konstantin Maslov, Konstantin Maslov, Qifa Zhou, Qifa Zhou, K. Kirk Shung, K. Kirk Shung, Lihong V. Wang, Lihong V. Wang, } "Label-free optical-resolution photoacoustic endomicroscopy in vivo", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 932332 (11 March 2015); doi: 10.1117/12.2080224;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89399
Deposited By: Tony Diaz
Deposited On:06 Sep 2018 16:45
Last Modified:03 Mar 2020 13:01

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