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Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome

Wong, Terence T. W. and Zhang, Ruiying and Hsu, Hsun-Chia and Maslov, Konstantin I. and Shi, Junhui and Chen, Ruimin and Shung, K. Kirk and Zhou, Qifa and Wang, Lihong V. (2018) Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome. In: Photons Plus Ultrasound: Imaging and Sensing 2018. Proceedings of SPIE. No.10494. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 104942N. ISBN 9781510614734.

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In biomedical imaging, all optical techniques face a fundamental trade-off between spatial resolution and tissue penetration. Therefore, obtaining an organelle-level resolution image of a whole organ has remained a challenging and yet appealing scientific pursuit. Over the past decade, optical microscopy assisted by mechanical sectioning or chemical clearing of tissue has been demonstrated as a powerful technique to overcome this dilemma, one of particular use in imaging the neural network. However, this type of techniques needs lengthy special preparation of the tissue specimen, which hinders broad application in life sciences. Here, we propose a new label-free three-dimensional imaging technique, named microtomy-assisted photoacoustic microscopy (mPAM), for potentially imaging all biomolecules with 100% endogenous natural staining in whole organs with high fidelity. We demonstrate the first label-free mPAM, using UV light for label-free histology-like imaging, in whole organs (e.g., mouse brains), most of them formalin-fixed and paraffin- or agarose-embedded for minimal morphological deformation. Furthermore, mPAM with dual wavelength illuminations is also employed to image a mouse brain slice, demonstrating the potential for imaging of multiple biomolecules without staining. With visible light illumination, mPAM also shows its deep tissue imaging capability, which enables less slicing and hence reduces sectioning artifacts. mPAM could potentially provide a new insight for understanding complex biological organs.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Wong, Terence T. W.0000-0001-6399-758X
Zhang, Ruiying0000-0002-0092-0814
Maslov, Konstantin I.0000-0003-3408-8840
Shi, Junhui0000-0002-5741-2781
Chen, Ruimin0000-0002-5338-359X
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2018 Society of Photo-Optical Instrumentation Engineers.
Subject Keywords:Brain, photoacoustic imaging, microscopy, label-free, histology, microtome, whole-organ atlas
Series Name:Proceedings of SPIE
Issue or Number:10494
Record Number:CaltechAUTHORS:20180905-155148719
Persistent URL:
Official Citation:Terence T. W. Wong, Terence T. W. Wong, Ruiying Zhang, Ruiying Zhang, Hsun-Chia Hsu, Hsun-Chia Hsu, Konstantin I. Maslov, Konstantin I. Maslov, Junhui Shi, Junhui Shi, Ruimin Chen, Ruimin Chen, K. Kirk Shung, K. Kirk Shung, Qifa Zhou, Qifa Zhou, Lihong V. Wang, Lihong V. Wang, } "Whole-organ atlas imaged by label-free high-resolution photoacoustic microscopy assisted by a microtome", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104942N (19 February 2018); doi: 10.1117/12.2291056;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89404
Deposited By: Tony Diaz
Deposited On:06 Sep 2018 16:40
Last Modified:09 Mar 2020 13:19

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