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Modulation of Asymmetric Division Diversity through Cytokinin and SPEECHLESS Regulatory Interactions in the Arabidopsis Stomatal Lineage

Vatén, Anne and Soyars, Cara L. and Tarr, Paul T. and Nimchuk, Zachary L. and Bergmann, Dominique C. (2018) Modulation of Asymmetric Division Diversity through Cytokinin and SPEECHLESS Regulatory Interactions in the Arabidopsis Stomatal Lineage. Developmental Cell, 47 (1). pp. 53-66. ISSN 1534-5807. PMCID PMC6177308. http://resolver.caltech.edu/CaltechAUTHORS:20180906-125123454

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Abstract

Coordinated growth of organs requires communication among cells within and between tissues. In plants, leaf growth is largely dictated by the epidermis; here, asymmetric and self-renewing divisions of the stomatal lineage create two essential cell types—pavement cells and guard cells—in proportions reflecting inputs from local, systemic, and environmental cues. The transcription factor SPEECHLESS (SPCH) is the prime regulator of divisions, but whether and how it is influenced by external cues to provide flexible development is enigmatic. Here, we show that the phytohormone cytokinin (CK) can act as an endogenous signal to affect the extent and types of stomatal lineage divisions and forms a regulatory circuit with SPCH. Local domains of low CK signaling are created by SPCH-dependent cell-type-specific activity of two repressive type-A ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR16 and ARR17, and two secreted peptides, CLE9 and CLE10, which, together with SPCH, can customize epidermal cell-type composition.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.devcel.2018.08.007DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6177308/PubMed CentralArticle
Additional Information:© 2018 Elsevier Inc. Received 25 May 2017, Revised 6 June 2018, Accepted 8 August 2018, Available online 6 September 2018. We thank Professor T. Kakimoto for reagents and the members of the Bergmann lab for discussions and comments on the manuscript. Funding was provided by EMBO Postdoctoral fellowship ALTF 707-2012 to A.V., NSF IOS-1546837 to Z.L.N., and NIHRO1GM086632 to D.C.B. P.T.T. was supported by NIH R01GM104244 and Howard Hughes Medical Institute (HHMI)/Gordon and Betty Moore Foundation GBMF3406 (awarded to E. Meyerowitz). D.C.B. is an HHMI investigator. Author Contributions: A.V. designed and performed all experiments (except as noted below), analyzed results, performed statistical analysis, and wrote the manuscript. C.L.S. and Z.L.N. created cle9 and cle10 mutants. P.T.T. created and provided reporter lines and clones to enable manipulation of CK levels. D.C.B. analyzed results and wrote the manuscript. The authors declare no competing interests.
Funders:
Funding AgencyGrant Number
European Molecular Biology Organization (EMBO)ALTF 707-2012
NSFIOS-1546837
NIHR01GM086632
NIHR01GM104244
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationGBMF3406
Subject Keywords:stomatal development; meristemoid behavior; cytokinin signaling; peptide signaling; growth regulation; Arabidopsis; SPEECHLESS
PubMed Central ID:PMC6177308
Record Number:CaltechAUTHORS:20180906-125123454
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180906-125123454
Official Citation:Anne Vatén, Cara L. Soyars, Paul T. Tarr, Zachary L. Nimchuk, Dominique C. Bergmann, Modulation of Asymmetric Division Diversity through Cytokinin and SPEECHLESS Regulatory Interactions in the Arabidopsis Stomatal Lineage, Developmental Cell, Volume 47, Issue 1, 2018, Pages 53-66.e5, ISSN 1534-5807, https://doi.org/10.1016/j.devcel.2018.08.007. (http://www.sciencedirect.com/science/article/pii/S1534580718306725)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89414
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Sep 2018 00:07
Last Modified:05 Dec 2018 18:04

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