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Grueneisen relaxation photoacoustic microscopy in vivo

Ma, Jun and Shi, Junhui and Hai, Pengfei and Zhou, Yong and Wang, Lihong V. (2016) Grueneisen relaxation photoacoustic microscopy in vivo. Journal of Biomedical Optics, 21 (6). Art. No. 066005. ISSN 1083-3668. PMCID PMC4897030. doi:10.1117/1.JBO.21.6.066005.

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Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5  μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Shi, Junhui0000-0002-5741-2781
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers. Paper 160121RR received Feb. 26, 2016; accepted for publication May 19, 2016; published online Jun. 8, 2016. The authors appreciate Professor James Ballard’s help in editing this paper. The authors also thank Lidai Wang and Konstantin Maslov for discussions and technical help in the experiments. This work was supported in part by the National Institutes of Health, Grant Nos. DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), R01 EB016963, and R01 CA159959. L.V.W. has a financial interest in Microphotoacoustics, Inc. which, however, did not support this work.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 EB016963
NIHR01 CA159959
Subject Keywords:photoacoustic imaging; nonlinear microscopy; Grueneisen relaxation; optical sectioning
Issue or Number:6
PubMed Central ID:PMC4897030
Record Number:CaltechAUTHORS:20180906-160753296
Persistent URL:
Official Citation:Jun Ma, Jun Ma, Junhui Shi, Junhui Shi, Pengfei Hai, Pengfei Hai, Yong Zhou, Yong Zhou, Lihong V. Wang, Lihong V. Wang, } "Grueneisen relaxation photoacoustic microscopy in vivo," Journal of Biomedical Optics 21(6), 066005 (8 June 2016).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89438
Deposited By: Tony Diaz
Deposited On:07 Sep 2018 00:19
Last Modified:16 Nov 2021 00:35

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