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In vivo photoacoustic neuronal imaging of odor-evoked calcium signals in the drosophila brain

Zhang, Ruiying and Rao, Bin and Rong, Haoyang and Raman, Baranidharan and Wang, Lihong V. (2016) In vivo photoacoustic neuronal imaging of odor-evoked calcium signals in the drosophila brain. In: Photons Plus Ultrasound: Imaging and Sensing 2016. Proceedings of SPIE. No.9708. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 97082V. ISBN 9781628419429. https://resolver.caltech.edu/CaltechAUTHORS:20180907-104554103

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Abstract

Neural scientists can benefit greatly from imaging tools that can penetrate thick brain tissue. Compared with traditional optical microscopy methods, photoacoustic imaging can beat the optical diffusion limit and achieve such deep tissue imaging with high spatial resolution. In this study, we used an optical-resolution photoacoustic microscope to image the odor-evoked neuronal activities in a drosophila model. Drosophila brain neurons stably express GCaMP5G, a calcium-sensitive fluorescent protein whose optical absorption coefficient changes with calcium influx during action potentials. We recorded an ~20% odor-evoked fractional photoacoustic signal increase at all depths of the drosophila brain in vivo, with and without removal of the brain cuticle, at a recording rate of 1 kHz. Our results were confirmed by concurrent fluorescent recordings. Furthermore, by performing fast 2D scanning, we imaged the antenna lobe region, which is of particular interest in neuroscience, at a volumetric rate of ~1 Hz with a sub-neuron resolution of 3 m. Unlike optical imaging, which requires surgical removal of the scattering brain cuticle, our photoacoustic system can image through the cuticle and measure neuronal signals of the whole drosophila brain without invasive surgery, enabling minimal disturbance to the animal’s behaviors. In conclusion, we have demonstrated photoacoustic imaging of calcium signals in drosophila brains for the first time. Utilizing the deep imaging capability of photoacoustic tomography, our methods could potentially be extended to in vivo imaging of neuronal activities from deep brains in other animal models.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2235935DOIArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers.
Series Name:Proceedings of SPIE
Issue or Number:9708
Record Number:CaltechAUTHORS:20180907-104554103
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180907-104554103
Official Citation:Ruiying Zhang, Ruiying Zhang, Bin Rao, Bin Rao, Haoyang Rong, Haoyang Rong, Baranidharan Raman, Baranidharan Raman, Lihong V. Wang, Lihong V. Wang, } "In vivo photoacoustic neuronal imaging of odor-evoked calcium signals in the drosophila brain (Conference Presentation)", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97082V (27 April 2016); doi: 10.1117/12.2235935; https://doi.org/10.1117/12.2235935
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89463
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Sep 2018 03:23
Last Modified:03 Oct 2019 20:16

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