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Photoacoustic imaging of voltage signals

Rao, Bin and Zhang, Ruiying and Wang, Lihong V. (2016) Photoacoustic imaging of voltage signals. In: Photons Plus Ultrasound: Imaging and Sensing 2016. Proceedings of SPIE. No.9708. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 97081U. ISBN 9781628419429. http://resolver.caltech.edu/CaltechAUTHORS:20180907-104554204

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Abstract

Optical imaging of brain voltage signals is significantly limited in depth due to optical scattering and the absorptive property of brain tissue. Photoacoustic (PA) imaging promises to break this hard limit by utilizing both ballistic and diffused photons. To demonstrate the feasibility of PA, we used an in vivo mouse model. The brain cortex tissue was stained with dipicrylamine dye, electrically stimulated, and imaged with a customized dual-isosbestic-wavelength PA microscope (DIW-PAM). DIW-PAM separates voltage-induced PA signals from blood-induced PA signals and thereby allows recording the voltage response of mouse cortex tissue without interference from hemoglobin responses. The resting state PA voltage response signal exhibited a noise-like signal in the frequency domain. Upon 3 Hz electrical stimulation, the PA voltage response signal showed frequency peaks of 3.2 Hz and 6.3 Hz (Fig. 1). Although dipicrylamine dye is not fast enough for recording neuron action potentials, it served well for the purpose of this feasibility study. In conclusion, we successfully demonstrated in vivo photoacoustic imaging of mouse brain voltage signals for the first time. If a fast voltage-sensitive dye is available, using photoacoustic computed tomography (PACT) instead of PA microscopy could allow acquiring full-field PA action potential images at a speed limited only by the laser pulse repetition rate.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2211747DOIArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers.
Record Number:CaltechAUTHORS:20180907-104554204
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180907-104554204
Official Citation:Bin Rao, Bin Rao, Ruiying Zhang, Ruiying Zhang, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic imaging of voltage signals (Conference Presentation)", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97081U (27 April 2016); doi: 10.1117/12.2211747; https://doi.org/10.1117/12.2211747
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89464
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Sep 2018 03:25
Last Modified:08 Sep 2018 03:25

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