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FGF controls epithelial-mesenchymal transitions during gastrulation by regulating cell division and apicobasal polarity

Sun, Jingjing and Stathopoulos, Angelike (2018) FGF controls epithelial-mesenchymal transitions during gastrulation by regulating cell division and apicobasal polarity. Development, 145 (19). Art. No. dev161927. ISSN 0950-1991. http://resolver.caltech.edu/CaltechAUTHORS:20180910-090200528

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Abstract

To support tissue and organ development, cells transition between epithelial and mesenchymal states. Here, we have investigated how mesoderm cells change state in Drosophila embryos and whether fibroblast growth factor (FGF) signaling plays a role. During gastrulation, presumptive mesoderm cells invaginate, undergo an epithelial-to-mesenchymal state transition (EMT) and migrate upon the ectoderm. Our data show that EMT is a prolonged process in which adherens junctions progressively decrease in number throughout the migration of mesoderm cells. FGF influences adherens junction number and promotes mesoderm cell division, which we propose decreases cell-cell attachments to support slow EMT while retaining collective cell movement. We also found that, at the completion of migration, cells form a monolayer and undergo a reverse mesenchymal-to-epithelial transition (MET). FGF activity leads to accumulation of β-integrin Myospheroid basally and cell polarity factor Bazooka apically within mesoderm cells, thereby reestablishing apicobasal cell polarity in an epithelialized state in which cells express both E-Cadherin and N-Cadherin. In summary, FGF plays a dynamic role in supporting mesoderm cell development to ensure collective mesoderm cell movements, as well as proper differentiation of mesoderm cell types.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1242/dev.161927DOIArticle
ORCID:
AuthorORCID
Stathopoulos, Angelike0000-0001-6597-2036
Additional Information:© 2018 Published by The Company of Biologists Ltd. Received November 29, 2017; Accepted August 31, 2018; First posted online on 6 September 2018. Published 1 October 2018. We are grateful to H. Ashe, A. Michelson, E. Wieschaus and J. Zallen for sharing fly stocks and antibodies, and to Z. Ákos, F. Macabenta, A. McMahon, W. Supatto and N. Trisnadi for help with Drosophila genetics, sharing preliminary results and antibodies, imaging and tracking, comments on the manuscript, and modifications to scripts as well as helpful discussions. The authors declare no competing or financial interests. Author contributions: Conceptualization: J.S., A.S.; Methodology: J.S.; Validation: J.S., A.S.; Formal analysis: J.S., A.S.; Investigation: J.S.; Resources: J.S., A.S.; Data curation: J.S.; Writing - original draft: A.S.; Writing - review & editing: J.S., A.S.; Visualization: J.S.; Supervision: A.S.; Project administration: A.S.; Funding acquisition: A.S. This work was funded by the National Institutes of Health grant R35GM118146 to A.S. Deposited in PMC for release after 12 months.
Funders:
Funding AgencyGrant Number
NIHR35GM118146
Subject Keywords:Drosophila melanogaster; Epithelial-to-mesenchymal (EMT); Mesenchymal-to-epithelial (MET); Fibroblast growth factor (FGF); Adherens junction; Integrin; Bazooka
Record Number:CaltechAUTHORS:20180910-090200528
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180910-090200528
Official Citation:FGF controls epithelial-mesenchymal transitions during gastrulation by regulating cell division and apicobasal polarity. Jingjing Sun, Angelike Stathopoulos. Development 2018 145: dev161927 doi: 10.1242/dev.161927 Published 1 October 2018
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89476
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Sep 2018 17:01
Last Modified:16 Oct 2018 16:56

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