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Photoacoustic imaging of single circulating melanoma cells in vivo

Wang, Lidai and Yao, Junjie and Zhang, Ruiying and Xu, Song and Li, Guo and Zou, Jun and Wang, Lihong V. (2015) Photoacoustic imaging of single circulating melanoma cells in vivo. In: Photons Plus Ultrasound: Imaging and Sensing 2015. Proceedings of SPIE. No.9323. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 93230A. ISBN 9781628414134. https://resolver.caltech.edu/CaltechAUTHORS:20180911-103014208

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Abstract

Melanoma, one of the most common types of skin cancer, has a high mortality rate, mainly due to a high propensity for tumor metastasis. The presence of circulating tumor cells (CTCs) is a potential predictor for metastasis. Label-free imaging of single circulating melanoma cells in vivo provides rich information on tumor progress. Here we present photoacoustic microscopy of single melanoma cells in living animals. We used a fast-scanning optical-resolution photoacoustic microscope to image the microvasculature in mouse ears. The imaging system has sub-cellular spatial resolution and works in reflection mode. A fast-scanning mirror allows the system to acquire fast volumetric images over a large field of view. A 500-kHz pulsed laser was used to image blood and CTCs. Single circulating melanoma cells were imaged in both capillaries and trunk vessels in living animals. These high-resolution images may be used in early detection of CTCs with potentially high sensitivity. In addition, this technique enables in vivo study of tumor cell extravasation from a primary tumor, which addresses an urgent pre-clinical need.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.2076987DOIArticle
ORCID:
AuthorORCID
Zhang, Ruiying0000-0002-0092-0814
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2015 Society of Photo-Optical Instrumentation Engineers.
Subject Keywords:Photoacoustic imaging, circulating tumor cell, melanoma
Series Name:Proceedings of SPIE
Issue or Number:9323
Record Number:CaltechAUTHORS:20180911-103014208
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180911-103014208
Official Citation:Lidai Wang, Lidai Wang, Junjie Yao, Junjie Yao, Ruiying Zhang, Ruiying Zhang, Song Xu, Song Xu, Guo Li, Guo Li, Jun Zou, Jun Zou, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic imaging of single circulating melanoma cells in vivo", Proc. SPIE 9323, Photons Plus Ultrasound: Imaging and Sensing 2015, 93230A (11 March 2015); doi: 10.1117/12.2076987; https://doi.org/10.1117/12.2076987
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89521
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Sep 2018 20:01
Last Modified:09 Mar 2020 13:19

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