A Caltech Library Service

Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome

Yao, Junjie and Kaberniuk, Andrii A. and Li, Lei and Shcherbakova, Daria M. and Zhang, Ruiying and Wang, Lidai and Li, Guo and Verkhusha, Vladislav V. and Wang, Lihong V. (2016) Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome. In: Photons Plus Ultrasound: Imaging and Sensing 2016. Proceedings of SPIE. No.9708. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 97082U. ISBN 9781628419429.

[img] PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Optical imaging of genetically encoded probes has revolutionized biomedical studies by providing valuable information about targeted biological processes. Here, we report a novel imaging technique, termed reversibly switchable photoacoustic tomography (RS-PAT), which exhibits large penetration depth, high detection sensitivity, and super-resolution. RS-PAT combines advanced photoacoustic imaging techniques with, for the first time, a nonfluorescent photoswitchable bacterial phytochrome. This bacterial phytochrome is the most near-infrared shifted genetically encoded probe reported so far. Moreover, this bacterial phytochrome is reversibly photoconvertible between its far-red and near-infrared light absorption states. Taking maximum advantage of the powerful imaging capability of PAT and the unique photochemical properties of the phytochrome, RS-PAT has broken through both the optical diffusion limit for deep-tissue imaging and the optical diffraction limit for super-resolution photoacoustic microscopy. Specifically, with RS-PAT we have achieved an unprecedented detection sensitivity of ~2 μM, or as few as ~20 tumor cells, at a centimeter depth. Such high sensitivity is fully demonstrated in our study by monitoring tumor growth and metastasis at whole-body level with ~100 μm resolution. Moreover, our microscopic implementation of RS-PAT is capable of imaging mammalian cells with a sub-diffraction lateral resolution of ~140 nm and axial resolution of ~400 nm, which are respectively ~2-fold and ~75-fold finer than those of our conventional photoacoustic microscopy. Overall, RS-PAT is a new and promising imaging technology for studying biological processes at different length scales.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers. This work was sponsored by US National Institutes of Health (NIH) grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), 1S10 RR028864 and R01 CA159959.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIH1S10 RR028864
NIHR01 CA159959
Subject Keywords:Photoacoustic tomography; Photoacoustic microscopy; Genetic imaging; Super-resolution imaging; Deep tissue imaging; Reversibly switchable protein; Bacterial phytochrome; Near-infrared protein
Series Name:Proceedings of SPIE
Issue or Number:9708
Record Number:CaltechAUTHORS:20180911-140924685
Persistent URL:
Official Citation:Junjie Yao, Junjie Yao, Andrii A. Kaberniuk, Andrii A. Kaberniuk, Lei Li, Lei Li, Daria M. Shcherbakova, Daria M. Shcherbakova, Ruiying Zhang, Ruiying Zhang, Lidai Wang, Lidai Wang, Guo Li, Guo Li, Vladislav V. Verkhusha, Vladislav V. Verkhusha, Lihong V. Wang, Lihong V. Wang, } "Reversibly switchable photoacoustic tomography using a genetically encoded near-infrared phytochrome", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97082U (18 March 2016); doi: 10.1117/12.2229156;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89527
Deposited By: Tony Diaz
Deposited On:11 Sep 2018 23:22
Last Modified:03 Oct 2019 20:17

Repository Staff Only: item control page