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Bessel beam Grueneisen photoacoustic microscopy with extended depth of field

Shi, Junhui and Wang, Lidai and Noordam, Cedric and Wang, Lihong V. (2016) Bessel beam Grueneisen photoacoustic microscopy with extended depth of field. In: Photons Plus Ultrasound: Imaging and Sensing 2016. Proceedings of SPIE. No.9708. Society of Photo-Optical Instrumentation Engineers , Bellingham, WA, Art. No. 97083H. ISBN 9781628419429.

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The short focal depth of a Gaussian beam limits the volumetric imaging speed of optical resolution photoacoustic microscopy (OR-PAM). A Bessel beam, which is diffraction-free, provides a long focal depth, but its side-lobes may deteriorate image quality when the Bessel beam is directly employed to excite photoacoustic signals in ORPAM. Here, we present a nonlinear approach based on the Grueneisen relaxation effect to suppress the side-lobe artifacts in photoacoustic imaging. This method extends the focal depth of OR-PAM and speeds up volumetric imaging. We experimentally demonstrated a 1-mm focal depth with a 7-μm lateral resolution and volumetrically imaged a carbon fiber and red blood cell samples.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Shi, Junhui0000-0002-5741-2781
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers. The authors appreciate Prof. James Ballard’s help with editing the manuscript. The authors also thank Konstantin Maslov, Lei Li, Jinyang Liang, and Mohammadreza Nasiriavanaki for discussions and technical help in the experiments. This work was supported in part by National Institutes of Health grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), and R01 CA159959. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 CA159959
Subject Keywords:Photoacoustic, Bessel beam, Grueneisen relaxation, nonlinear microscopy
Series Name:Proceedings of SPIE
Issue or Number:9708
Record Number:CaltechAUTHORS:20180911-142432014
Persistent URL:
Official Citation:Junhui Shi, Junhui Shi, Lidai Wang, Lidai Wang, Cedric Noordam, Cedric Noordam, Lihong V. Wang, Lihong V. Wang, } "Bessel beam Grueneisen photoacoustic microscopy with extended depth of field", Proc. SPIE 9708, Photons Plus Ultrasound: Imaging and Sensing 2016, 97083H (15 March 2016); doi: 10.1117/12.2211198;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89528
Deposited By: Tony Diaz
Deposited On:11 Sep 2018 23:21
Last Modified:16 Nov 2021 00:36

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