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Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo

Zhou, Yong and Yao, Junjie and Maslov, Konstantin I. and Wang, Lihong V. (2014) Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo. Journal of Biomedical Optics, 19 (3). Art. No. 037001. ISSN 1083-3668. PMCID PMC3939437. https://resolver.caltech.edu/CaltechAUTHORS:20180911-164454823

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Abstract

Currently, laser fluence calibration is typically required for quantitative measurement of particle concentration in photoacoustic imaging. Here, we present a calibration-free method to quantify the absolute particle concentration by statistically analyzing photoacoustic signals. The proposed method is based on the fact that Brownian motion induces particle count fluctuation in the detection volume. If the count of particles in the detection volume is assumed to follow the Poisson distribution, its expected value can be calculated by the photoacoustic signal mean and variance. We first derived a theoretical model for photoacoustic signals. Then, we applied our method to quantitative measurement of different concentrations of various particles, including red blood cells. Finally, we performed in vivo experiments to demonstrate the potential of our method in biological applications. The experimental results agreed well with the predictions from the theoretical model suggesting that our method can be used for noninvasive measurement of absolute particle concentrations in deep tissue without fluence calibration.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/1.jbo.19.3.037001DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3939437/PubMed CentralArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 130767R received Oct. 24, 2013; revised manuscript received Jan. 27, 2014; accepted for publication Jan. 28, 2014; published online Mar. 3, 2014. The authors would like to thank James Ballard and Seema Dahlheimer for this paper editing. We also appreciate technical assistance and useful discussions with Jinyang Liang, Chi Zhang, Chenghung Yeh, Lidai Wang, Arie Krumholz and Da-Kang Yao. This work was sponsored in part by National Institutes of Health Grants Nos. DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), R01 EB016963, R01 CA134539, R01 CA157277, and R01 CA159959. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work. K.M. has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHR01 EB016963
NIHR01 CA134539
NIHR01 CA157277
NIHR01 CA159959
Subject Keywords:photoacoustic imaging; Poisson distribution; particle concentration; calibration free; red blood cell
Issue or Number:3
PubMed Central ID:PMC3939437
Record Number:CaltechAUTHORS:20180911-164454823
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180911-164454823
Official Citation:Yong Zhou, Yong Zhou, Junjie Yao, Junjie Yao, Konstantin I. Maslov, Konstantin I. Maslov, Lihong V. Wang, Lihong V. Wang, } "Calibration-free absolute quantification of particle concentration by statistical analyses of photoacoustic signals in vivo," Journal of Biomedical Optics 19(3), 037001 (3 March 2014). https://doi.org/10.1117/1.JBO.19.3.037001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89547
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Sep 2018 14:31
Last Modified:03 Oct 2019 20:17

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