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Label-free photoacoustic tomography of whole mouse brain structures ex vivo

Li, Lei and Xia, Jun and Li, Guo and Garcia-Uribe, Alejandro and Sheng, Qiwei and Anastasio, Mark A. and Wang, Lihong V. (2016) Label-free photoacoustic tomography of whole mouse brain structures ex vivo. Neurophotonics, 3 (3). Art. No. 035001. ISSN 2329-423X. PMCID PMC5696384.

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Capitalizing on endogenous hemoglobin contrast, photoacoustic-computed tomography (PACT), a deep-tissue high-resolution imaging modality, has drawn increasing interest in neuroimaging. However, most existing studies are limited to functional imaging on the cortical surface and the deep brain structural imaging capability of PACT has never been demonstrated. Here, we explicitly studied the limiting factors of deep brain PACT imaging. We found that the skull distorted the acoustic signal and blood suppressed the structural contrast from other chromophores. When the two effects are mitigated, PACT can potentially provide high-resolution label-free imaging of structures in the entire mouse brain. With 100-μm in-plane resolution, we can clearly identify major structures of the brain, which complements magnetic resonance microscopy for imaging small-animal brain structures. Spectral PACT studies indicate that structural contrasts mainly originate from cytochrome distribution and that the presence of lipid sharpens the image contrast; brain histology results provide further validation. The feasibility of imaging the structure of the brain in vivo is also discussed. Our results demonstrate that PACT is a promising modality for both structural and functional brain imaging.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Anastasio, Mark A.0000-0002-3192-4172
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 16030PR received May 19, 2016; accepted for publication Jul. 6, 2016; published online Jul. 26, 2016. The authors appreciate Prof. James Ballard’s close reading of the manuscript, and thank Ernie Gonzales, Dr. Junjie Yao, Dr. Chi Zhang, and Chiye Li for their useful discussion and technical assistance. This work was sponsored in part by the National Institutes of Health (NIH) Grant Nos. DP1 EB016986 (NIH Director’s Pioneer Award), R01 CA186567 (NIH Director’s Transformative Research Award), U01 NS090579 (BRAIN Initiative), and R01 EB016963. L. V. Wang has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work. L. V. Wang has a financial interest in Endra, Inc., and Microphotoacoustics, Inc., which, however, did not support this work. The other authors declare no competing financial interests.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 CA186567
NIHU01 NS090579
NIHR01 EB016963
Subject Keywords:photoacoustic-computed tomography; brain structural imaging; deep brain imaging; label free; spectral imaging
Issue or Number:3
PubMed Central ID:PMC5696384
Record Number:CaltechAUTHORS:20180911-164455001
Persistent URL:
Official Citation:Lei Li, Lei Li, Jun Xia, Jun Xia, Guo Li, Guo Li, Alejandro Garcia-Uribe, Alejandro Garcia-Uribe, Qiwei Sheng, Qiwei Sheng, Mark A. Anastasio, Mark A. Anastasio, Lihong V. Wang, Lihong V. Wang, } "Label-free photoacoustic tomography of whole mouse brain structures ex vivo," Neurophotonics 3(3), 035001 (26 July 2016).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89549
Deposited By: George Porter
Deposited On:12 Sep 2018 14:29
Last Modified:03 Oct 2019 20:17

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