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Handheld optical-resolution photoacoustic microscopy

Lin, Li and Zhang, Pengfei and Xu, Song and Shi, Junhui and Li, Lei and Yao, Junjie and Wang, Lidai and Zou, Jun and Wang, Lihong V. (2017) Handheld optical-resolution photoacoustic microscopy. Journal of Biomedical Optics, 22 (4). Art. No. 041002. ISSN 1083-3668. PMCID PMC5075719.

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Optical-resolution photoacoustic microscopy (OR-PAM) offers label-free in vivo imaging with high spatial resolution by acoustically detecting optical absorption contrasts via the photoacoustic effect. We developed a compact handheld OR-PAM probe for fast photoacoustic imaging. Different from benchtop microscopes, the handheld probe provides flexibility in imaging various anatomical sites. Resembling a cup in size, the probe uses a two-axis water-immersible microelectromechanical system mirror to scan both the illuminating optical beam and resultant acoustic beam. The system performance was tested in vivo by imaging the capillary bed in a mouse ear and both the capillary bed and a mole on a human volunteer.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Lin, Li0000-0002-0517-8436
Zhang, Pengfei0000-0002-4226-1394
Shi, Junhui0000-0002-5741-2781
Li, Lei0000-0001-6164-2646
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE). Paper 160264SSR received Apr. 21, 2016; accepted for publication Jun. 9, 2016; published online Oct. 24, 2016. This work was funded by the National Institutes of Health (NIH) (DP1-EB016986, R01-CA186567, and R01-CA159959) and National Science Foundation (NSF) (1255930). The authors thank Professor James Ballard for his close reading of the manuscript. L. V. Wang has a financial interest in Microphotoacoustics, Inc., which, however, did not support this work.
Funding AgencyGrant Number
Subject Keywords:photoacoustic handheld probe; optical-resolution photoacoustic microscopy; two-axis microelectromechanical system mirror; confocal scanning; human skin imaging
Issue or Number:4
PubMed Central ID:PMC5075719
Record Number:CaltechAUTHORS:20180911-164455137
Persistent URL:
Official Citation:Li Lin, Li Lin, Pengfei Zhang, Pengfei Zhang, Song Xu, Song Xu, Junhui Shi, Junhui Shi, Lei Li, Lei Li, Junjie Yao, Junjie Yao, Lidai Wang, Lidai Wang, Jun Zou, Jun Zou, Lihong V. Wang, Lihong V. Wang, } "Handheld optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 22(4), 041002 (24 October 2016). .
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89550
Deposited By: George Porter
Deposited On:12 Sep 2018 14:25
Last Modified:29 Oct 2020 21:47

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