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Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy

Yao, Junjie and Huang, Chih-Hsien and Martel, Catherine and Maslov, Konstantin I. and Wang, Lidai and Yang, Joon-Mo and Gao, Liang and Randolph, Gwendalyn and Zou, Jun and Wang, Lihong V. (2013) Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy. In: Photons Plus Ultrasound: Imaging and Sensing 2013. Proceedings of SPIE. No.8581. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 858127. ISBN 9780819493507.

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By offering images with high spatial resolution and unique optical absorption contrast, optical-resolution photoacoustic microscopy (OR-PAM) has gained increasing attention in biomedical research. Recent developments in OR-PAM have improved its imaging speed, but have sacrificed either the detection sensitivity or field of view or both. We have developed a wide-field fast-scanning OR-PAM by using a water-immersible MEMS scanning mirror (MEMS-ORPAM). Made of silicon with a gold coating, the MEMS mirror plate can reflect both optical and acoustic beams. Because it uses an electromagnetic driving force, the whole MEMS scanning system can be submerged in water. In MEMS-ORPAM, the optical and acoustic beams are confocally configured and simultaneously steered, which ensures uniform detection sensitivity. A B-scan imaging speed as high as 400 Hz can be achieved over a 3 mm scanning range. A diffraction-limited lateral resolution of 2.4 μm in water and a maximum imaging depth of 1.1 mm in soft tissue have been experimentally determined. Using the system, we imaged the flow dynamics of both red blood cells and carbon particles in a mouse ear in vivo. By using Evans blue dye as the contrast agent, we also imaged the flow dynamics of lymphatic vessels in a mouse tail in vivo. The results show that MEMS-OR-PAM could be a powerful tool for studying highly dynamic and time-sensitive biological phenomena.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Maslov, Konstantin I.0000-0003-3408-8840
Zou, Jun0000-0002-9543-6135
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). The authors would like to thank Prof. James Ballard for manuscript editing. We also appreciate technical assistance and useful discussions with Yan Liu. This research was supported by the National Institutes of Health Grants DP1 EB016986 (NIH Director’s Pioneer Award), R01 EB008085, R01 CA134539, U54 CA136398, R01 CA157277, R01 CA159959. L.V.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funding AgencyGrant Number
NIHDP1 EB016986
NIHR01 EB008085
NIHR01 CA134539
NIHU54 CA136398
NIHR01 CA157277
NIHR01 CA159959
Subject Keywords:Optical-resolution photoacoustic microscopy, MEMS scanning mirror, blood flow dynamic imaging, lymphatic flow dynamic imaging, functional brain imaging
Series Name:Proceedings of SPIE
Issue or Number:8581
Record Number:CaltechAUTHORS:20180914-101020280
Persistent URL:
Official Citation:Junjie Yao, Junjie Yao, Chih-Hsien Huang, Chih-Hsien Huang, Catherine Martel, Catherine Martel, Konstantin I. Maslov, Konstantin I. Maslov, Lidai Wang, Lidai Wang, Joon-Mo Yang, Joon-Mo Yang, Liang Gao, Liang Gao, Gwendalyn Randolph, Gwendalyn Randolph, Jun Zou, Jun Zou, Lihong V. Wang, Lihong V. Wang, } "Water-Immersible MEMS scanning mirror designed for wide-field fast-scanning photoacoustic microscopy", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858127 (4 March 2013); doi: 10.1117/12.2005669;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89649
Deposited By: George Porter
Deposited On:14 Sep 2018 22:26
Last Modified:09 Mar 2020 13:19

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