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Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo

Cai, Xin and Zhang, Yu and Li, Li and Choi, Sung-Wook and MacEwan, Matthew R. and Yao, Junjie and Kim, Chulhong and Xia, Younan and Wang, Lihong V. (2013) Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo. In: Photons Plus Ultrasound: Imaging and Sensing 2013. Proceedings of SPIE. No.8581. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 858128. ISBN 9780819493507.

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It is a challenge to non-invasively visualize in vivo the neovascularization in a three-dimensional (3D) scaffold with high spatial resolution and deep penetration depth. Here we used photoacoustic microscopy (PAM) to chronically monitor neovascularization in an inverse opal scaffold implanted in a mouse model for up to six weeks. The neovasculature was observed to develop gradually in the same mouse. These blood vessels not only grew on top of the implanted scaffold but also penetrated into the scaffold. The PAM system offered a lateral resolution of ~45 μm and a penetration depth of ~3 mm into the scaffold/tissue construct. By using the 3D PAM data, we further quantified the vessel area as a function of time.

Item Type:Book Section
Related URLs:
URLURL TypeDescription
Zhang, Yu0000-0001-8938-1927
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE). Our work was also sponsored by NIH grants R01 EB000712, R01 EB008085, R01 CA140220, R01 CA157277, R01 CA159959, U54 CA136398, and DP1 EB016986-NIH Director's Pioneer Award. L.V.W. has a financial interest in Microphotoacoustics, Inc., and Endra, Inc., which, however, did not support this work. Others claim no competing financial interests.
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 CA140220
NIHR01 CA157277
NIHR01 CA159959
NIHU54 CA136398
NIHDP1 EB016986
Subject Keywords:Three-dimensional scaffold; photoacoustic microscopy; tissue engineering; neovascularization; biomedical imaging
Series Name:Proceedings of SPIE
Issue or Number:8581
Record Number:CaltechAUTHORS:20180917-094548927
Persistent URL:
Official Citation:Xin Cai, Xin Cai, Yu Zhang, Yu Zhang, Li Li, Li Li, Sung-Wook Choi, Sung-Wook Choi, Matthew R. MacEwan, Matthew R. MacEwan, Junjie Yao, Junjie Yao, Chulhong Kim, Chulhong Kim, Younan Xia, Younan Xia, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic microscopy of neovascularization in three-dimensional porous scaffolds in vivo", Proc. SPIE 8581, Photons Plus Ultrasound: Imaging and Sensing 2013, 858128 (4 March 2013); doi: 10.1117/12.2005236;
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89667
Deposited By: George Porter
Deposited On:17 Sep 2018 19:41
Last Modified:03 Oct 2019 20:18

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