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Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii

Story, Randall M. and Li, Hong and Abelson, John N. (2001) Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii. Proceedings of the National Academy of Sciences of the United States of America, 98 (4). pp. 1465-1470. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:STOpnas01

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Abstract

We have determined the structure of a DEAD box putative RNA helicase from the hyperthermophile Methanococcus jannaschii, Like other helicases, the protein contains two alpha/beta domains, each with a recA-like topology. Unlike other helicases, the protein exists as a dimer in the crystal. Through an interaction that resembles the dimer interface of insulin, the amino-terminal domain's 7-strand beta -sheet is extended to 14 strands across the two molecules. Motifs conserved in the DEAD box family cluster in the cleft between domains, and many of their functions can he deduced by mutational data and by comparison with other helicase structures. Several lines of evidence suggest that motif III Ser-Ala-Thr may be involved in binding RNA.


Item Type:Article
Additional Information:© 2001 by the National Academy of Sciences. Contributed by John N. Abelson, December 4, 2000. We thank members of the Rees and Bjorkman labs at California Institute of Technology for their assistance, advice, and use of their equipment, as well as Thomas Earnest and coworkers at the advanced light source synchrotron of Lawrence Berkeley National Laboratory. This work was supported by National Institutes of Health Grant GM32637 (to J.N.A.). Data deposition: The atomic coordinates have been deposited in the Protein Data Bank, www.rcsb.org (PDB ID code 1HV8). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:INITIATION-FACTOR 4A; RNA HELICASE; ESCHERICHIA-COLI; ATP HYDROLYSIS; DIFFRACTION DATA; PCRA HELICASE; DOMAIN; TRANSLATION; COMPLEXES; BINDING
Issue or Number:4
Record Number:CaltechAUTHORS:STOpnas01
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:STOpnas01
Alternative URL:http://dx.doi.org/10.1073/pnas.98.4.1465
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:8970
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Oct 2007
Last Modified:02 Oct 2019 23:56

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