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Fabricating multifunctional microbubbles and nanobubbles for concurrent ultrasound and photoacoustic imaging

Qin, Ruogu and Xu, Jeff and Xu, Ronald and Kim, Chulhong and Wang, Lihong V. (2010) Fabricating multifunctional microbubbles and nanobubbles for concurrent ultrasound and photoacoustic imaging. In: Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue II. Proceedings of SPIE. No.7567. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 75670L. ISBN 9780819479631. https://resolver.caltech.edu/CaltechAUTHORS:20180918-140708062

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Abstract

Background: Clinical ultrasound (US) uses ultrasonic scattering contrast to characterize subcutaneous anatomic structures. Photoacoustic (PA) imaging detects the functional properties of thick biological tissue with high optical contrast. In the case of image-guided cancer ablation therapy, simultaneous US and PA imaging can be useful for intraoperative assessment of tumor boundaries and ablation margins. In this regard, accurate co-registration between imaging modalities and high sensitivity to cancer cells are important. Methods: We synthesized poly-lactic-co-glycolic acid (PLGA) microbubbles (MBs) and nanobubbles (NBs) encapsulating India ink or indocyanine green (ICG). Multiple tumor simulators were fabricated by entrapping ink MBs or NBs at various concentrations in gelatin phantoms for simultaneous US and PA imaging. MBs and NBs were also conjugated with CC49 antibody to target TAG-72, a human glycoprotein complex expressed in many epithelial-derived cancers. Results: Accurate co-registration and intensity correlation were observed in US and PA images of MB and NB tumor simulators. MBs and NBs conjugating with CC49 effectively bound with over-expressed TAG-72 in LS174T colon cancer cell cultures. ICG was also encapsulated in MBs and NBs for the potential to integrate US, PA, and fluorescence imaging. Conclusions: Multifunctional MBs and NBs can be potentially used as a general contrast agent for multimodal intraoperative imaging of tumor boundaries and therapeutic margins.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.841281DOIArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by grants from the National Institutes of Health (R01 EB000712, R01 EB008085, and U54 CA136398 — the Network for Translational Research — to L.V.W.) and the Department of Defense Breast Cancer Research Program (W81XWH-07 to R. X.). L.V.W. has a financial interest in Microphotoacoustics, Inc. and in Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHU54 CA136398
Department of Defense Breast Cancer Research ProgramW81XWH-07
Subject Keywords:Ultrasound, photoacoustic, intraoperative, microbubbles, nanobubbles, contrast agent, cancer ablation
Series Name:Proceedings of SPIE
Issue or Number:7567
Record Number:CaltechAUTHORS:20180918-140708062
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180918-140708062
Official Citation:Ruogu Qin, Ruogu Qin, Jeff Xu, Jeff Xu, Ronald Xu, Ronald Xu, Chulhong Kim, Chulhong Kim, Lihong V. Wang, Lihong V. Wang, } "Fabricating multifunctional microbubbles and nanobubbles for concurrent ultrasound and photoacoustic imaging", Proc. SPIE 7567, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue II, 75670L (25 February 2010); doi: 10.1117/12.841281; https://doi.org/10.1117/12.841281
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89709
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Sep 2018 22:49
Last Modified:03 Oct 2019 20:18

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