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In vivo, label-free photoacoustic microscopy of the anterior segment of the mouse eye

Rao, Bin and Hu, Song and Li, Li and Maslov, Konstantin and Wang, Lihong V. (2010) In vivo, label-free photoacoustic microscopy of the anterior segment of the mouse eye. In: Photons Plus Ultrasound: Imaging and Sensing 2010. Proceedings of SPIE. No.7564. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 75643E. ISBN 9780819479600. http://resolver.caltech.edu/CaltechAUTHORS:20180918-140708592

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Abstract

Both iris fluorescein angiography (IFA) and indocyanine green angiography (ICGA) provide ophthalmologists imaging tools in studying the microvasculature structure and hemodynamics of the anterior segment of the eye in normal and diseased status. However, a non-invasive, endogenous imaging modality is preferable for the monitoring of hemodynamics of the iris microvasculature. We investigated the in vivo, label-free ocular anterior segment imaging with photo-acoustic microscopy (PAM) in mouse eyes. We demonstrated the unique advantage of endogenous contrast that is not available in both IFA and ICGA. The laser radiation was maintained within the ANSI laser safety limit. The in vivo, label-free nature of our imaging technology has the potential for ophthalmic applications.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.843052DOIArticle
ORCID:
AuthorORCID
Rao, Bin0000-0001-6494-3110
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). The mouse ocular anterior segment imaging demonstration done on the original OR-PAM system was contributed in equal measure by Bin Rao and Song Hu. The HS-ORPAM system and mouse ocular anterior segment imaging demonstration were contributed in equal measure by Bin Rao and Li Li. The research was supported by the National Institutes of Health grants R01 EB000712, EB000712A2S1, R01 EB00071207S2, R01 EB008085, R01 CA113453901, U54 CA136398, and 5P60 DK02057933. L.W. has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB000712A2S1
NIHR01 EB00071207S2
NIHR01 EB008085
NIHR01 CA113453901
NIHU54 CA136398
NIH5P60 DK02057933
Subject Keywords:photoacoustic microscopy, anterior segment of eye, iris imaging
Record Number:CaltechAUTHORS:20180918-140708592
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20180918-140708592
Official Citation:Bin Rao, Bin Rao, Song Hu, Song Hu, Li Li, Li Li, Konstantin Maslov, Konstantin Maslov, Lihong V. Wang, Lihong V. Wang, } "In vivo label-free photoacoustic microscopy of the anterior segment of the mouse eye", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75643E (23 February 2010); doi: 10.1117/12.843052; https://doi.org/10.1117/12.843052
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89714
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Sep 2018 23:06
Last Modified:19 Sep 2018 23:06

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