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In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging

Kim, Chulhong and Song, Kwang Hyun and Wang, Lihong V. (2010) In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging. In: Photons Plus Ultrasound: Imaging and Sensing 2010. Proceedings of SPIE. No.7564. Society of Photo-optical Instrumentation Engineers (SPIE) , Bellingham, WA, Art. No. 75642W. ISBN 9780819479600. https://resolver.caltech.edu/CaltechAUTHORS:20180918-140708965

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Abstract

The purpose of this study is to map non-invasively sentinel lymph nodes (SLNs) and lymphatic vessels of rats in vivo using FDA-approved indocyanine green (ICG) and two non-ionizing imaging modalities: volumetric spectroscopic photoacoustic (PA) imaging, which measures optical absorption, and planar fluorescence imaging, which measures fluorescent emission. SLNs and lymphatic vessels were clearly visible after a 0.2 ml-intradermal-injection of 1 mM ICG in both imaging systems. We also imaged deeply positioned lymph nodes in vivo by layering biological tissues on top of rats. These two modalities, when used together with ICG, have the potential to map SLNs in axillary staging and to study tumor metastasis in breast cancer patients.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1117/12.841964DOIArticle
ORCID:
AuthorORCID
Wang, Lihong V.0000-0001-9783-4383
Additional Information:© 2010 Society of Photo-Optical Instrumentation Engineers (SPIE). This work was supported in part by grants from National Institutes of Health (R01 EB000712, R01 EB008085, R01 NS46214, and U54 CA136398 -- the Network for Translational Research -- to L.V.W.). L.V.W. has a financial interest in Endra, Inc., which, however, did not support this work.
Funders:
Funding AgencyGrant Number
NIHR01 EB000712
NIHR01 EB008085
NIHR01 NS46214
NIHU54 CA136398
Subject Keywords:Photoacoustic tomography, planar fluorescence imaging, sentinel lymph node biopsy, breast cancer, axillary lymph node dissection
Series Name:Proceedings of SPIE
Issue or Number:7564
Record Number:CaltechAUTHORS:20180918-140708965
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20180918-140708965
Official Citation:Chulhong Kim, Chulhong Kim, Kwang Hyun Song, Kwang Hyun Song, Lihong V. Wang, Lihong V. Wang, } "In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: three-dimensional photoacoustic imaging and planar fluorescence imaging", Proc. SPIE 7564, Photons Plus Ultrasound: Imaging and Sensing 2010, 75642W (23 February 2010); doi: 10.1117/12.841964; https://doi.org/10.1117/12.841964
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:89718
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Sep 2018 23:20
Last Modified:03 Oct 2019 20:18

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